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| | <StructureSection load='4mer' size='340' side='right'caption='[[4mer]], [[Resolution|resolution]] 2.41Å' scene=''> | | <StructureSection load='4mer' size='340' side='right'caption='[[4mer]], [[Resolution|resolution]] 2.41Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mer]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MER FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mer]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MER FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M5005_Spy1730, SPy_2034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mer OCA], [https://pdbe.org/4mer PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mer RCSB], [https://www.ebi.ac.uk/pdbsum/4mer PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mer ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mer OCA], [http://pdbe.org/4mer PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mer RCSB], [http://www.ebi.ac.uk/pdbsum/4mer PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mer ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q99XU0_STRP1 Q99XU0_STRP1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Micrococcus scarlatinae klein 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bjorck, L]] | + | [[Category: Streptococcus pyogenes]] |
| - | [[Category: Frick, M I]] | + | [[Category: Bjorck L]] |
| - | [[Category: Happonen, L]] | + | [[Category: Frick M-I]] |
| - | [[Category: Malmstrom, J]] | + | [[Category: Happonen L]] |
| - | [[Category: Streicher, W]] | + | [[Category: Malmstrom J]] |
| - | [[Category: Wikstrom, M]] | + | [[Category: Streicher W]] |
| - | [[Category: Wisniewska, M]] | + | [[Category: Wikstrom M]] |
| - | [[Category: Helix]]
| + | [[Category: Wisniewska M]] |
| - | [[Category: Tetramer]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
Q99XU0_STRP1
Publication Abstract from PubMed
Streptococcus pyogenes is a significant bacterial pathogen in the human population. The importance of virulence factors for the survival and colonization of S. pyogenes is well established, and many of these factors are exposed to the extracellular environment enabling bacterial interactions with the host. In the present study we quantitatively analyzed and compared S. pyogenes proteins in the growth medium of a strain that is virulent to mice, with a non-virulent strain. Particularly one of these proteins was present at significantly higher levels in stationary growth medium from the virulent strain. We determined the three-dimensional structure of the protein that showed a unique tetrameric organization composed of four helix-loop-helix motifs. Affinity pull-down mass spectrometry analysis in human plasma demonstrated that the protein interacts with histidine-rich glycoprotein (HRG), and the name sHIP (streptococcal Histidine-rich glycoprotein Interacting Protein) is therefore proposed. HRG has antibacterial activity, and when challenged by HRG, sHIP was found to rescue S. pyogenes bacteria. This and the finding that patients with invasive S. pyogenes infection respond with antibody production against sHIP, suggest a role for the protein in S. pyogenes pathogenesis.
Functional and Structural Properties of a Novel Protein and Virulence Factor (sHIP) in Streptococcus pyogenes.,Wisniewska M, Happonen L, Kahn F, Varjosalo M, Malmstrom L, Rosenberger G, Karlsson C, Cazzamali G, Pozdnyakova I, Frick IM, Bjorck L, Streicher W, Malmstrom J, Wikstrom M J Biol Chem. 2014 May 13. pii: jbc.M114.565978. PMID:24825900[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wisniewska M, Happonen L, Kahn F, Varjosalo M, Malmstrom L, Rosenberger G, Karlsson C, Cazzamali G, Pozdnyakova I, Frick IM, Bjorck L, Streicher W, Malmstrom J, Wikstrom M. Functional and Structural Properties of a Novel Protein and Virulence Factor (sHIP) in Streptococcus pyogenes. J Biol Chem. 2014 May 13. pii: jbc.M114.565978. PMID:24825900 doi:http://dx.doi.org/10.1074/jbc.M114.565978
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