7jku
From Proteopedia
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==Crystal structure of a four-tetrad, parallel, and K+ stabilized Tetrahymena thermophila telomeric G-quadruplex== | ==Crystal structure of a four-tetrad, parallel, and K+ stabilized Tetrahymena thermophila telomeric G-quadruplex== | ||
| - | <StructureSection load='7jku' size='340' side='right'caption='[[7jku]]' scene=''> | + | <StructureSection load='7jku' size='340' side='right'caption='[[7jku]], [[Resolution|resolution]] 1.97Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JKU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7jku]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JKU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jku OCA], [https://pdbe.org/7jku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jku RCSB], [https://www.ebi.ac.uk/pdbsum/7jku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jku ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jku OCA], [https://pdbe.org/7jku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jku RCSB], [https://www.ebi.ac.uk/pdbsum/7jku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jku ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | G-quadruplexes (GQs) are non-canonical DNA structures composed of stacks of stabilized G-tetrads. GQs play an important role in a variety of biological processes and may form at telomeres and oncogene promoters among other genomic locations. Here, we investigate nine variants of telomeric DNA from Tetrahymena thermophila with the repeat (TTGGGG)n. Biophysical data indicate that the sequences fold into stable four-tetrad GQs which adopt multiple conformations according to native PAGE. Excitingly, we solved the crystal structure of two variants, TET25 and TET26. The two variants differ by the presence of a 3'-T yet adopt different GQ conformations. TET25 forms a hybrid [3 + 1] GQ and exhibits a rare 5'-top snapback feature. Consequently, TET25 contains four loops: three lateral (TT, TT, and GTT) and one propeller (TT). TET26 folds into a parallel GQ with three TT propeller loops. To the best of our knowledge, TET25 and TET26 are the first reported hybrid and parallel four-tetrad unimolecular GQ structures. The results presented here expand the repertoire of available GQ structures and provide insight into the intricacy and plasticity of the 3D architecture adopted by telomeric repeats from T. thermophila and GQs in general. | ||
| + | |||
| + | The first crystal structures of hybrid and parallel four-tetrad intramolecular G-quadruplexes.,Beseiso D, Chen EV, McCarthy SE, Martin KN, Gallagher EP, Miao J, Yatsunyk LA Nucleic Acids Res. 2022 Mar 21;50(5):2959-2972. doi: 10.1093/nar/gkac091. PMID:35212369<ref>PMID:35212369</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7jku" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Tetrahymena thermophila]] | ||
[[Category: Beseiso D]] | [[Category: Beseiso D]] | ||
[[Category: Chen EV]] | [[Category: Chen EV]] | ||
Revision as of 09:13, 28 December 2022
Crystal structure of a four-tetrad, parallel, and K+ stabilized Tetrahymena thermophila telomeric G-quadruplex
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