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| | <StructureSection load='4mj3' size='340' side='right'caption='[[4mj3]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='4mj3' size='340' side='right'caption='[[4mj3]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mj3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_rhodesiae_js60 Mycobacterium rhodesiae js60]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MJ3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mj3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_rhodesiae_JS60 Mycolicibacterium rhodesiae JS60]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MJ3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mj3 OCA], [https://pdbe.org/4mj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mj3 RCSB], [https://www.ebi.ac.uk/pdbsum/4mj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mj3 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MycrhDRAFT_3907 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=931627 Mycobacterium rhodesiae JS60])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mj3 OCA], [http://pdbe.org/4mj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mj3 RCSB], [http://www.ebi.ac.uk/pdbsum/4mj3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mj3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G4I2J6_MYCRH G4I2J6_MYCRH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Dehalogenase|Dehalogenase]] | + | *[[Dehalogenase 3D structures|Dehalogenase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Haloalkane dehalogenase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mycobacterium rhodesiae js60]] | + | [[Category: Mycolicibacterium rhodesiae JS60]] |
| - | [[Category: Fung, H]] | + | [[Category: Fung H]] |
| - | [[Category: Gadd, M S]] | + | [[Category: Gadd MS]] |
| - | [[Category: Guss, J M]] | + | [[Category: Guss JM]] |
| - | [[Category: Matthews, J M]] | + | [[Category: Matthews JM]] |
| - | [[Category: Dehalogenase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
G4I2J6_MYCRH
Publication Abstract from PubMed
Haloalkane dehalogenases (HLDs) catalyse the hydrolysis of haloalkanes to alcohols, offering a biological solution for toxic haloalkane industrial wastes. Hundreds of putative HLD genes have been identified in bacterial genomes, but relatively few enzymes have been characterised. We identified two novel HLDs in the genome of Mycobacterium rhodesiae strain JS60, an isolate from an organochlorine-contaminated site: DmrA and DmrB. Both recombinant enzymes were active against C2-C6 haloalkanes, with a preference for brominated linear substrates. However, DmrA had higher activity against a wider range of substrates. The kinetic parameters of DmrA with 4-bromobutyronitrile as a substrate were Km = 1.9 +/- 0.2 mM, kcat = 3.1 +/- 0.2 s(-1) . DmrB showed the highest activity against 1-bromohexane. DmrA is monomeric, whereas DmrB is tetrameric. We determined the crystal structure of selenomethionyl DmrA to 1.7 A resolution. A spacious active site and alternate conformations of a methionine side-chain in the slot access tunnel may contribute to the broad substrate activity of DmrA. We show that M. rhodesiae JS60 can utilise 1-iodopropane, 1-iodobutane and 1-bromobutane as sole carbon and energy sources. This ability appears to be conferred predominantly through DmrA, which shows significantly higher levels of upregulation in response to haloalkanes than DmrB.
Biochemical and biophysical characterisation of haloalkane dehalogenases DmrA and DmrB in Mycobacterium strain JS60 and their role in growth on haloalkanes.,Fung HK, Gadd MS, Drury TA, Cheung S, Guss JM, Coleman NV, Matthews JM Mol Microbiol. 2015 Aug;97(3):439-53. doi: 10.1111/mmi.13039. Epub 2015 May 20. PMID:25899475[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Fung HK, Gadd MS, Drury TA, Cheung S, Guss JM, Coleman NV, Matthews JM. Biochemical and biophysical characterisation of haloalkane dehalogenases DmrA and DmrB in Mycobacterium strain JS60 and their role in growth on haloalkanes. Mol Microbiol. 2015 Aug;97(3):439-53. doi: 10.1111/mmi.13039. Epub 2015 May 20. PMID:25899475 doi:http://dx.doi.org/10.1111/mmi.13039
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