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| | ==The crystal structure of a M20 family metallo-carboxypeptidase Sso-CP2 from Sulfolobus solfataricus== | | ==The crystal structure of a M20 family metallo-carboxypeptidase Sso-CP2 from Sulfolobus solfataricus== |
| - | <StructureSection load='4mmo' size='340' side='right' caption='[[4mmo]], [[Resolution|resolution]] 2.34Å' scene=''> | + | <StructureSection load='4mmo' size='340' side='right'caption='[[4mmo]], [[Resolution|resolution]] 2.34Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mmo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MMO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mmo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MMO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSO2737 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mmo OCA], [https://pdbe.org/4mmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mmo RCSB], [https://www.ebi.ac.uk/pdbsum/4mmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mmo ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mmo OCA], [http://pdbe.org/4mmo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mmo RCSB], [http://www.ebi.ac.uk/pdbsum/4mmo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mmo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q97V97_SACS2 Q97V97_SACS2] |
| - | The genome of the crenarchaeon Sulfolobus solfataricus P2 contains 2,992,245 bp on a single chromosome and encodes 2,977 proteins and many RNAs. One-third of the encoded proteins have no detectable homologs in other sequenced genomes. Moreover, 40% appear to be archaeal-specific, and only 12% and 2.3% are shared exclusively with bacteria and eukarya, respectively. The genome shows a high level of plasticity with 200 diverse insertion sequence elements, many putative nonautonomous mobile elements, and evidence of integrase-mediated insertion events. There are also long clusters of regularly spaced tandem repeats. Different transfer systems are used for the uptake of inorganic and organic solutes, and a wealth of intracellular and extracellular proteases, sugar, and sulfur metabolizing enzymes are encoded, as well as enzymes of the central metabolic pathways and motility proteins. The major metabolic electron carrier is not NADH as in bacteria and eukarya but probably ferredoxin. The essential components required for DNA replication, DNA repair and recombination, the cell cycle, transcriptional initiation and translation, but not DNA folding, show a strong eukaryal character with many archaeal-specific features. The results illustrate major differences between crenarchaea and euryarchaea, especially for their DNA replication mechanism and cell cycle processes and their translational apparatus.
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| - | The complete genome of the crenarchaeon Sulfolobus solfataricus P2.,She Q, Singh RK, Confalonieri F, Zivanovic Y, Allard G, Awayez MJ, Chan-Weiher CC, Clausen IG, Curtis BA, De Moors A, Erauso G, Fletcher C, Gordon PM, Heikamp-de Jong I, Jeffries AC, Kozera CJ, Medina N, Peng X, Thi-Ngoc HP, Redder P, Schenk ME, Theriault C, Tolstrup N, Charlebois RL, Doolittle WF, Duguet M, Gaasterland T, Garrett RA, Ragan MA, Sensen CW, Van der Oost J Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7835-40. Epub 2001 Jun 26. PMID:11427726<ref>PMID:11427726</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 4mmo" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Sulso]] | + | [[Category: Large Structures]] |
| - | [[Category: Bauvois, C]] | + | [[Category: Saccharolobus solfataricus P2]] |
| - | [[Category: Borel, F]] | + | [[Category: Bauvois C]] |
| - | [[Category: Demarez, M]] | + | [[Category: Borel F]] |
| - | [[Category: Dupuy, J]] | + | [[Category: Demarez M]] |
| - | [[Category: Durisotti, V]] | + | [[Category: Dupuy J]] |
| - | [[Category: Dutoit, R]] | + | [[Category: Durisotti V]] |
| - | [[Category: Elder, D Van]]
| + | [[Category: Dutoit R]] |
| - | [[Category: Legrain, C]] | + | [[Category: Legrain C]] |
| - | [[Category: Carboxypeptidase]] | + | [[Category: Van Elder D]] |
| - | [[Category: Hydrolase]]
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| - | [[Category: M20 family peptidase]]
| + | |
| - | [[Category: Metal-binding hydrolase]]
| + | |
| - | [[Category: Metallo protein]]
| + | |
| - | [[Category: Metalloprotease]]
| + | |
| - | [[Category: Protease]]
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