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1jiw
From Proteopedia
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[[Image:1jiw.jpg|left|200px]] | [[Image:1jiw.jpg|left|200px]] | ||
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'''Crystal structure of the APR-APRin complex''' | '''Crystal structure of the APR-APRin complex''' | ||
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[[Category: Gray, R D.]] | [[Category: Gray, R D.]] | ||
[[Category: Hege, T.]] | [[Category: Hege, T.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:16:40 2008'' | |
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Revision as of 18:16, 2 May 2008
Crystal structure of the APR-APRin complex
Overview
Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.
About this Structure
1JIW is a Protein complex structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:11445573 Page seeded by OCA on Fri May 2 21:16:40 2008
