7xxj
From Proteopedia
(Difference between revisions)
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==Echo 18 incubated with FcRn at pH5.5== | ==Echo 18 incubated with FcRn at pH5.5== | ||
| - | <StructureSection load='7xxj' size='340' side='right'caption='[[7xxj]]' scene=''> | + | <StructureSection load='7xxj' size='340' side='right'caption='[[7xxj]], [[Resolution|resolution]] 3.33Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XXJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7xxj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echovirus_E18 Echovirus E18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XXJ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xxj OCA], [https://pdbe.org/7xxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xxj RCSB], [https://www.ebi.ac.uk/pdbsum/7xxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xxj ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xxj OCA], [https://pdbe.org/7xxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xxj RCSB], [https://www.ebi.ac.uk/pdbsum/7xxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xxj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Virus-receptor interactions determine viral host range and tissue tropism. CD55 and human neonatal Fc receptor (FcRn) were found to be the binding and uncoating receptors for some of the echovirus-related enterovirus species B serotypes in our previous study. Echovirus 18 (E18), as a member of enterovirus species B, is a significant causative agent of aseptic meningitis and viral encephalitis in children. However, it does not use CD55 as a critical host factor. We conducted CRISPR/Cas9 knockout screening to determine the receptors and entry mechanisms and identified FcRn working as a dual-function receptor for E18. Knockout of FCGRT and B2M, which encode the two subunits of FcRn, prevented infection by E18 and other echoviruses in the same physiological cluster. We then elucidated the underlying molecular mechanism of receptor recognition by E18 using cryogenic electron microscopy. The binding of the FCGRT subunit to the canyon region rotates the residues around the pocket, triggering the release of the pocket factor as observed for other enterovirus species B members. IMPORTANCE E18 is a member of enterovirus species B. As one of the most common enterovirus serotypes in nonpolio enterovirus detection, it easily infects children and causes various clinical symptoms. Aseptic meningitis and viral encephalitis are the most commonly reported syndromes associated with E18. No effective antiviral drugs or approved vaccines are available. Previous studies showed that CD55 and FcRn were the binding and uncoating receptors for some echoviruses. However, we found that CD55 is not the critical host factor for E18. Thus, we want to determine the receptors and elucidate the entry mechanism of E18. Our findings reveal that FcRn is a two-in-one attachment-uncoating receptor for E18. | ||
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| + | Human FcRn Is a Two-in-One Attachment-Uncoating Receptor for Echovirus 18.,Chen X, Qu X, Liu C, Zhang Y, Zhang G, Han P, Duan Y, Li Q, Wang L, Ruan W, Wang P, Wei W, Gao GF, Zhao X, Xie Z mBio. 2022 Aug 30;13(4):e0116622. doi: 10.1128/mbio.01166-22. Epub 2022 Jul 5. PMID:35862785<ref>PMID:35862785</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7xxj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Echovirus E18]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Liu CC]] |
| + | [[Category: Qu X]] | ||
Revision as of 07:48, 11 January 2023
Echo 18 incubated with FcRn at pH5.5
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