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| | ==Crystal Structure of Saccharomyces cerevisiae Upc2 Transcription Factor fused with T4 Lysozyme== | | ==Crystal Structure of Saccharomyces cerevisiae Upc2 Transcription Factor fused with T4 Lysozyme== |
| - | <StructureSection load='4n9n' size='340' side='right' caption='[[4n9n]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='4n9n' size='340' side='right'caption='[[4n9n]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4n9n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N9N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4n9n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N9N FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UPC2, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9n OCA], [https://pdbe.org/4n9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n9n RCSB], [https://www.ebi.ac.uk/pdbsum/4n9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9n ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9n OCA], [http://pdbe.org/4n9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n9n RCSB], [http://www.ebi.ac.uk/pdbsum/4n9n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9n ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UPC2_YEAST UPC2_YEAST]] Transcription factor that is involved in activation of anaerobic genes such as DAN/TIR cell wall mannoprotein genes and YML083c. Appears to bind to anaerobic response elements (AR1) with the consensus sequence 5'-TCGTTYAG-3' present in the promoter regions of DAN/TIR genes. Involved in sterol uptake and regulation of the sterol biosynthesis. Binds to sterol regulatory elements (SRE) with the consensus sequence 5'-TCGTATA-3' present in ERG2 and ERG3 promoters. May be involved in down-regulation of CWP2 during anaerobic adaptation.<ref>PMID:11238402</ref> <ref>PMID:11292809</ref> <ref>PMID:11533229</ref> <ref>PMID:12077145</ref> <ref>PMID:12673627</ref> | + | [https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> [https://www.uniprot.org/uniprot/UPC2_YEAST UPC2_YEAST] Transcription factor that is involved in activation of anaerobic genes such as DAN/TIR cell wall mannoprotein genes and YML083c. Appears to bind to anaerobic response elements (AR1) with the consensus sequence 5'-TCGTTYAG-3' present in the promoter regions of DAN/TIR genes. Involved in sterol uptake and regulation of the sterol biosynthesis. Binds to sterol regulatory elements (SRE) with the consensus sequence 5'-TCGTATA-3' present in ERG2 and ERG3 promoters. May be involved in down-regulation of CWP2 during anaerobic adaptation.<ref>PMID:11238402</ref> <ref>PMID:11292809</ref> <ref>PMID:11533229</ref> <ref>PMID:12077145</ref> <ref>PMID:12673627</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpt4]] | + | [[Category: Escherichia virus T4]] |
| - | [[Category: Im, Y J]] | + | [[Category: Large Structures]] |
| - | [[Category: Yang, H]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Ergosterol binding]] | + | [[Category: Im YJ]] |
| - | [[Category: Fungal nuclear receptor-like transcription factor]] | + | [[Category: Yang H]] |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
ENLYS_BPT4 Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.[1] UPC2_YEAST Transcription factor that is involved in activation of anaerobic genes such as DAN/TIR cell wall mannoprotein genes and YML083c. Appears to bind to anaerobic response elements (AR1) with the consensus sequence 5'-TCGTTYAG-3' present in the promoter regions of DAN/TIR genes. Involved in sterol uptake and regulation of the sterol biosynthesis. Binds to sterol regulatory elements (SRE) with the consensus sequence 5'-TCGTATA-3' present in ERG2 and ERG3 promoters. May be involved in down-regulation of CWP2 during anaerobic adaptation.[2] [3] [4] [5] [6]
References
- ↑ Moussa SH, Kuznetsov V, Tran TA, Sacchettini JC, Young R. Protein determinants of phage T4 lysis inhibition. Protein Sci. 2012 Apr;21(4):571-82. doi: 10.1002/pro.2042. Epub 2012 Mar 2. PMID:22389108 doi:http://dx.doi.org/10.1002/pro.2042
- ↑ Abramova NE, Cohen BD, Sertil O, Kapoor R, Davies KJ, Lowry CV. Regulatory mechanisms controlling expression of the DAN/TIR mannoprotein genes during anaerobic remodeling of the cell wall in Saccharomyces cerevisiae. Genetics. 2001 Mar;157(3):1169-77. PMID:11238402
- ↑ Abramova N, Sertil O, Mehta S, Lowry CV. Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae. J Bacteriol. 2001 May;183(9):2881-7. PMID:11292809 doi:http://dx.doi.org/10.1128/JB.183.9.2881-2887.2001
- ↑ Vik A, Rine J. Upc2p and Ecm22p, dual regulators of sterol biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol. 2001 Oct;21(19):6395-405. PMID:11533229
- ↑ Wilcox LJ, Balderes DA, Wharton B, Tinkelenberg AH, Rao G, Sturley SL. Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast. J Biol Chem. 2002 Sep 6;277(36):32466-72. Epub 2002 Jun 20. PMID:12077145 doi:http://dx.doi.org/10.1074/jbc.M204707200
- ↑ Ter Linde JJ, Regnacq M, Steensma HY. Transcriptional regulation of YML083c under aerobic and anaerobic conditions. Yeast. 2003 Apr 15;20(5):439-54. PMID:12673627 doi:http://dx.doi.org/10.1002/yea.975
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