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| | <StructureSection load='4ngh' size='340' side='right'caption='[[4ngh]], [[Resolution|resolution]] 2.68Å' scene=''> | | <StructureSection load='4ngh' size='340' side='right'caption='[[4ngh]], [[Resolution|resolution]] 2.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ngh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ngh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NGH FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DIV:D-ISOVALINE'>DIV</scene>, <scene name='pdbligand=LYV:O-PHOSPHONO-L-SERYL-N-[(3S)-3-AMINO-3-CARBOXYPROPYL]GLYCINAMIDE'>LYV</scene>, <scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DIV:D-ISOVALINE'>DIV</scene>, <scene name='pdbligand=LYV:O-PHOSPHONO-L-SERYL-N-[(3S)-3-AMINO-3-CARBOXYPROPYL]GLYCINAMIDE'>LYV</scene>, <scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nhc|4nhc]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ngh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngh OCA], [https://pdbe.org/4ngh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ngh RCSB], [https://www.ebi.ac.uk/pdbsum/4ngh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngh OCA], [http://pdbe.org/4ngh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ngh RCSB], [http://www.ebi.ac.uk/pdbsum/4ngh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Irimia, A]] | + | [[Category: Irimia A]] |
| - | [[Category: Wilson, I A]] | + | [[Category: Wilson IA]] |
| - | [[Category: 4e10 fab]]
| + | |
| - | [[Category: Antibody-epitope complex]]
| + | |
| - | [[Category: Beta-sandwich]]
| + | |
| - | [[Category: Gp41 hiv-1]]
| + | |
| - | [[Category: Hydrocarbon staple]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin fold]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Hydrocarbon stapling can restore bioactive alpha-helical structure to natural peptides, yielding research tools and prototype therapeutics to dissect and target protein interactions. Here we explore the capacity of peptide stapling to generate high-fidelity, protease-resistant mimics of antigenic structures for vaccine development. HIV-1 has been refractory to vaccine technologies thus far, although select human antibodies can broadly neutralize HIV-1 by targeting sequences of the gp41 juxtamembrane fusion apparatus. To develop candidate HIV-1 immunogens, we generated and characterized stabilized alpha-helices of the membrane-proximal external region (SAH-MPER) of gp41. SAH-MPER peptides were remarkably protease resistant and bound to the broadly neutralizing 4E10 and 10E8 antibodies with high affinity, recapitulating the structure of the MPER epitope when differentially engaged by the two anti-HIV Fabs. Thus, stapled peptides may provide a new opportunity to develop chemically stabilized antigens for vaccination.
Stapled HIV-1 peptides recapitulate antigenic structures and engage broadly neutralizing antibodies.,Bird GH, Irimia A, Ofek G, Kwong PD, Wilson IA, Walensky LD Nat Struct Mol Biol. 2014 Nov 24. doi: 10.1038/nsmb.2922. PMID:25420104[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bird GH, Irimia A, Ofek G, Kwong PD, Wilson IA, Walensky LD. Stapled HIV-1 peptides recapitulate antigenic structures and engage broadly neutralizing antibodies. Nat Struct Mol Biol. 2014 Nov 24. doi: 10.1038/nsmb.2922. PMID:25420104 doi:http://dx.doi.org/10.1038/nsmb.2922
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