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| | ==Crystal structure of the catalytic core of RRP6 from Trypanosoma brucei, mutant C496S== | | ==Crystal structure of the catalytic core of RRP6 from Trypanosoma brucei, mutant C496S== |
| - | <StructureSection load='4nlc' size='340' side='right' caption='[[4nlc]], [[Resolution|resolution]] 2.15Å' scene=''> | + | <StructureSection load='4nlc' size='340' side='right'caption='[[4nlc]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4nlc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tryb2 Tryb2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NLC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4nlc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NLC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nlb|4nlb]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nlc OCA], [https://pdbe.org/4nlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nlc RCSB], [https://www.ebi.ac.uk/pdbsum/4nlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nlc ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tb927.4.1630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=999953 TRYB2])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nlc OCA], [http://pdbe.org/4nlc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nlc RCSB], [http://www.ebi.ac.uk/pdbsum/4nlc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nlc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q581R8_TRYB2 Q581R8_TRYB2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Tryb2]] | + | [[Category: Large Structures]] |
| - | [[Category: Barbosa, R L]] | + | [[Category: Trypanosoma brucei brucei TREU927]] |
| - | [[Category: Guimaraes, B G]] | + | [[Category: Barbosa RL]] |
| - | [[Category: 3'-5' exoribonuclease]] | + | [[Category: Guimaraes BG]] |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
Q581R8_TRYB2
Publication Abstract from PubMed
RRP6 is a 3'-5' exoribonuclease associated to the eukaryotic exosome, a multiprotein complex essential for various RNA processing and degradation pathways. In Trypanosoma brucei, RRP6 associates with the exosome in stoichiometric amounts and was localized in both cytoplasm and nucleus, in contrast to yeast Rrp6 which is exclusively nuclear. Here we report the biochemical and structural characterization of T. brucei RRP6 (TbRRP6) and its interaction with the so-called T. brucei Exosome Associated Protein 3 (TbEAP3), a potential orthologue of the yeast Rrp6 interacting protein, Rrp47. Recombinant TbEAP3 is a thermo stable homodimer in solution, however it forms a heterodimeric complex with TbRRP6 with 1ratio1 stoichiometry. The crystallographic structure of the TbRRP6 catalytic core exposes for the first time the native catalytic site of this RNase and also reveals a disulfide bond linking two helices of the HRDC domain. RNA degradation assays show the distributive exoribonuclease activity of TbRRP6 and novel findings regarding the structural range of its RNA substrates. TbRRP6 was able to degrade single and double-stranded RNAs and also RNA substrates containing stem-loops including those with 3' stem-loop lacking single-stranded extensions. Finally, association with TbEAP3 did not significantly interfere with the TbRRP6 catalytic activity in vitro.
RRP6 from Trypanosoma brucei: Crystal Structure of the Catalytic Domain, Association with EAP3 and Activity towards Structured and Non-Structured RNA Substrates.,Barbosa RL, Legrand P, Wien F, Pineau B, Thompson A, Guimaraes BG PLoS One. 2014 Feb 18;9(2):e89138. doi: 10.1371/journal.pone.0089138. eCollection, 2014. PMID:24558481[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Barbosa RL, Legrand P, Wien F, Pineau B, Thompson A, Guimaraes BG. RRP6 from Trypanosoma brucei: Crystal Structure of the Catalytic Domain, Association with EAP3 and Activity towards Structured and Non-Structured RNA Substrates. PLoS One. 2014 Feb 18;9(2):e89138. doi: 10.1371/journal.pone.0089138. eCollection, 2014. PMID:24558481 doi:http://dx.doi.org/10.1371/journal.pone.0089138
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