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| | <StructureSection load='4nno' size='340' side='right'caption='[[4nno]], [[Resolution|resolution]] 1.17Å' scene=''> | | <StructureSection load='4nno' size='340' side='right'caption='[[4nno]], [[Resolution|resolution]] 1.17Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4nno]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NNO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4NNO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4nno]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NNO FirstGlance]. <br> |
| | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4k3v|4k3v]], [[4nnp|4nnp]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nno OCA], [https://pdbe.org/4nno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nno RCSB], [https://www.ebi.ac.uk/pdbsum/4nno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nno ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAV0631 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4nno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nno OCA], [http://pdbe.org/4nno PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nno RCSB], [http://www.ebi.ac.uk/pdbsum/4nno PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nno ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H3JTS7_STAAM A0A0H3JTS7_STAAM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Staam]] | + | [[Category: Staphylococcus aureus subsp. aureus Mu50]] |
| - | [[Category: Ahuja, S]] | + | [[Category: Ahuja S]] |
| - | [[Category: Rouge, L]] | + | [[Category: Rouge L]] |
| - | [[Category: Sudhamsu, J]] | + | [[Category: Sudhamsu J]] |
| - | [[Category: Abc superfamily atp binding cassette transporter]]
| + | |
| - | [[Category: Manganese transporter]]
| + | |
| - | [[Category: Mrsa]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
A0A0H3JTS7_STAAM
Publication Abstract from PubMed
Bacterial ATP-binding cassette (ABC) importers play critical roles in nutrient acquisition and are potential antibacterial targets. However, structural bases for their inhibition are poorly defined. These pathways typically rely on substrate binding proteins (SBPs), which are essential for substrate recognition, delivery, and transporter function. We report the crystal structure of a Staphylococcus aureus SBP for Mn(II), termed MntC, in complex with FabC1, a potent antibody inhibitor of the MntABC pathway. This pathway is essential and highly expressed during S. aureus infection and facilitates the import of Mn(II), a critical cofactor for enzymes that detoxify reactive oxygen species (ROS). Structure-based functional studies indicate that FabC1 sterically blocks a structurally conserved surface of MntC, preventing its interaction with the MntB membrane importer and increasing wild-type S. aureus sensitivity to oxidative stress by more than 10-fold. The results define an SBP blocking mechanism as the basis for ABC importer inhibition by an engineered antibody fragment.
Structural analysis of bacterial ABC transporter inhibition by an antibody fragment.,Ahuja S, Rouge L, Swem DL, Sudhamsu J, Wu P, Russell SJ, Alexander MK, Tam C, Nishiyama M, Starovasnik MA, Koth CM Structure. 2015 Apr 7;23(4):713-23. doi: 10.1016/j.str.2015.01.020. Epub 2015 Mar, 5. PMID:25752540[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ahuja S, Rouge L, Swem DL, Sudhamsu J, Wu P, Russell SJ, Alexander MK, Tam C, Nishiyama M, Starovasnik MA, Koth CM. Structural analysis of bacterial ABC transporter inhibition by an antibody fragment. Structure. 2015 Apr 7;23(4):713-23. doi: 10.1016/j.str.2015.01.020. Epub 2015 Mar, 5. PMID:25752540 doi:http://dx.doi.org/10.1016/j.str.2015.01.020
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