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| | ==Extended-Synaptotagmin 2, C2A- and C2B-domains, calcium bound== | | ==Extended-Synaptotagmin 2, C2A- and C2B-domains, calcium bound== |
| - | <StructureSection load='4npk' size='340' side='right' caption='[[4npk]], [[Resolution|resolution]] 2.55Å' scene=''> | + | <StructureSection load='4npk' size='340' side='right'caption='[[4npk]], [[Resolution|resolution]] 2.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4npk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NPK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4npk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NPK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4npj|4npj]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4npk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npk OCA], [https://pdbe.org/4npk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4npk RCSB], [https://www.ebi.ac.uk/pdbsum/4npk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4npk ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ESYT2, extended-synaptotagmin 2, FAM62B, KIAA1228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4npk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npk OCA], [http://pdbe.org/4npk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4npk RCSB], [http://www.ebi.ac.uk/pdbsum/4npk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4npk ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN]] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref> | + | [https://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4npk" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4npk" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Synaptotagmin|Synaptotagmin]] |
| | + | *[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Rizo, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Tomchick, D R]] | + | [[Category: Rizo J]] |
| - | [[Category: Xu, J]]
| + | [[Category: Tomchick DR]] |
| - | [[Category: C2 domain]] | + | [[Category: Xu J]] |
| - | [[Category: Calcium/phospholipid binding protein]] | + | |
| - | [[Category: Er to plasma membrane]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Membrane traffic]]
| + | |
| - | [[Category: Plasma membrane]]
| + | |
| - | [[Category: Protein targeting]]
| + | |
| Structural highlights
Function
ESYT2_HUMAN May play a role as calcium-regulated intrinsic membrane protein.[1]
Publication Abstract from PubMed
Contacts between the endoplasmic reticulum and the plasma membrane involve extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins with multiple C2 domains. One of the tandem C2 domains of E-Syt2 is predicted to bind Ca2+, but no Ca2+-dependent function has been attributed to this protein. We have determined the crystal structures of the tandem C2 domains of E-Syt2 in the absence and presence of Ca2+ and analyzed their Ca2+-binding properties by nuclear magnetic resonance spectroscopy. Our data reveal an unexpected V-shaped structure with a rigid orientation between the two C2 domains that is not substantially altered by Ca2+. The E-Syt2 C2A domain binds up to four Ca2+ ions, whereas the C2B domain does not bind Ca2+. These results suggest that E-Syt2 performs an as yet unidentified Ca2+-dependent function through its C2A domain and uncover fundamental differences between the properties of the tandem C2 domains of E-Syts and synaptotagmins.
Structure and Ca-Binding Properties of the Tandem C Domains of E-Syt2.,Xu J, Bacaj T, Zhou A, Tomchick DR, Sudhof TC, Rizo J Structure. 2013 Dec 24. pii: S0969-2126(13)00461-9. doi:, 10.1016/j.str.2013.11.011. PMID:24373768[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Min SW, Chang WP, Sudhof TC. E-Syts, a family of membranous Ca2+-sensor proteins with multiple C2 domains. Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3823-8. Epub 2007 Feb 28. PMID:17360437 doi:http://dx.doi.org/0611725104
- ↑ Xu J, Bacaj T, Zhou A, Tomchick DR, Sudhof TC, Rizo J. Structure and Ca-Binding Properties of the Tandem C Domains of E-Syt2. Structure. 2013 Dec 24. pii: S0969-2126(13)00461-9. doi:, 10.1016/j.str.2013.11.011. PMID:24373768 doi:http://dx.doi.org/10.1016/j.str.2013.11.011
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