8e3i

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m (Protected "8e3i" [edit=sysop:move=sysop])
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'''Unreleased structure'''
 
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The entry 8e3i is ON HOLD
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==CRYO-EM STRUCTURE OF the human MPSF IN COMPLEX WITH THE AUUAAA poly(A) signal==
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<StructureSection load='8e3i' size='340' side='right'caption='[[8e3i]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8e3i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E3I FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e3i OCA], [https://pdbe.org/8e3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e3i RCSB], [https://www.ebi.ac.uk/pdbsum/8e3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e3i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The polyadenylation signal (PAS) is a key sequence element for 3'-end cleavage and polyadenylation of messenger RNA precursors (pre-mRNAs). This hexanucleotide motif is recognized by the mammalian polyadenylation specificity factor (mPSF), consisting of CPSF160, WDR33, CPSF30, and Fip1 subunits. Recent studies have revealed how the AAUAAA PAS, the most frequently observed PAS, is recognized by mPSF. We report here the structure of human mPSF in complex with the AUUAAA PAS, the second most frequently identified PAS. Conformational differences are observed for the A1 and U2 nucleotides in AUUAAA compared to the A1 and A2 nucleotides in AAUAAA, while the binding modes of the remaining 4 nt are essentially identical. The 5' phosphate of U2 moves by 2.6 A and the U2 base is placed near the six-membered ring of A2 in AAUAAA, where it makes two hydrogen bonds with zinc finger 2 (ZF2) of CPSF30, which undergoes conformational changes as well. We also attempted to determine the binding modes of two rare PAS hexamers, AAGAAA and GAUAAA, but did not observe the RNA in the cryo-electron microscopy density. The residues in CPSF30 (ZF2 and ZF3) and WDR33 that recognize PAS are disordered in these two structures.
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Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L.
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Molecular basis for the recognition of the AUUAAA polyadenylation signal by mPSF.,Gutierrez PA, Wei J, Sun Y, Tong L RNA. 2022 Nov;28(11):1534-1541. doi: 10.1261/rna.079322.122. Epub 2022 Sep 6. PMID:36130077<ref>PMID:36130077</ref>
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Description: CryoEM structure of mPSF in complex with the non-canonical PAS AUUAAA
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Tong, L]]
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<div class="pdbe-citations 8e3i" style="background-color:#fffaf0;"></div>
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[[Category: Gutierrez, P.A]]
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== References ==
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[[Category: Wei, J]]
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<references/>
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[[Category: Sun, Y]]
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Gutierrez PA]]
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[[Category: Sun Y]]
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[[Category: Tong L]]
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[[Category: Wei J]]

Revision as of 07:33, 18 January 2023

CRYO-EM STRUCTURE OF the human MPSF IN COMPLEX WITH THE AUUAAA poly(A) signal

PDB ID 8e3i

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