8du7
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Room-temperature serial synchrotron crystallography (SSX) structure of apo PTP1B== | ==Room-temperature serial synchrotron crystallography (SSX) structure of apo PTP1B== | ||
| - | <StructureSection load='8du7' size='340' side='right'caption='[[8du7]]' scene=''> | + | <StructureSection load='8du7' size='340' side='right'caption='[[8du7]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DU7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[8du7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DU7 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8du7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8du7 OCA], [https://pdbe.org/8du7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8du7 RCSB], [https://www.ebi.ac.uk/pdbsum/8du7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8du7 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8du7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8du7 OCA], [https://pdbe.org/8du7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8du7 RCSB], [https://www.ebi.ac.uk/pdbsum/8du7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8du7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PTN1_HUMAN PTN1_HUMAN] Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion.<ref>PMID:21135139</ref> <ref>PMID:22169477</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but obtaining sufficiently large protein crystals is a common hurdle. Serial synchrotron crystallography (SSX) helps to address this hurdle by allowing the use of many medium- to small-sized crystals. Here, a recently introduced serial sample-support chip system has been used to obtain the first SSX structure of a human phosphatase, specifically protein tyrosine phosphatase 1B (PTP1B) in the unliganded (apo) state. In previous apo room-temperature structures, the active site and allosteric sites adopted alternate conformations, including open and closed conformations of the active-site WPD loop and of a distal allosteric site. By contrast, in our SSX structure the active site is best fitted with a single conformation, but the distal allosteric site is best fitted with alternate conformations. This observation argues for additional nuance in interpreting the nature of allosteric coupling in this protein. Overall, our results illustrate the promise of serial methods for room-temperature crystallography, as well as future avant-garde crystallography experiments, for PTP1B and other proteins. | ||
| + | |||
| + | Room-temperature serial synchrotron crystallography of the human phosphatase PTP1B.,Sharma S, Ebrahim A, Keedy DA Acta Crystallogr F Struct Biol Commun. 2023 Jan 1;79(Pt 1):23-30. doi: , 10.1107/S2053230X22011645. Epub 2023 Jan 1. PMID:36598353<ref>PMID:36598353</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8du7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ebrahim A]] | [[Category: Ebrahim A]] | ||
[[Category: Keedy DA]] | [[Category: Keedy DA]] | ||
[[Category: Sharma S]] | [[Category: Sharma S]] | ||
Revision as of 07:47, 18 January 2023
Room-temperature serial synchrotron crystallography (SSX) structure of apo PTP1B
| |||||||||||
