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2kxd

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Revision as of 08:07, 18 January 2023

The structure of SH3-F2

Structural highlights

2kxd is a 1 chain structure with sequence from Gallus gallus and Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPTN1_CHICK Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.

Publication Abstract from PubMed

For the further elucidation of structural and dynamic principles of protein self-organization and protein-ligand interactions the design of new chimeric protein SH3-F2 was made and genetically engineered construct was created. The SH3-F2 amino acid sequence consists of polyproline ligand mgAPPLPPYSA, GG linker and the sequence of spectrin SH3 domain circular permutant S19-P20s. Structural and dynamics properties of the protein were studied by high-resolution NMR. According to NMR data the tertiary structure of the chimeric protein SH3-F2 has the topology which is typical of SH3 domains in the complex with the ligand, forming polyproline type II helix, located in the conservative region of binding in the orientation II. The polyproline ligand closely adjoins with the protein globule and is stabilized by hydrophobic interactions. However the interaction of ligand and the part of globule relative to SH3 domain is not too large because the analysis of protein dynamic characteristics points to the low amplitude, high-frequency ligand tumbling in relation to the slow intramolecular motions of the main globule. The constructed chimera permits to carry out further structural and thermodynamic investigations of polyproline helix properties and its interaction with regulatory domains.

[NMR structure and dynamics of the chimeric protein SH3-F2].,Kutyshenko VP, Gushchina LV, Khristoforov VS, Prokhorov DA, Timchenko MA, Kudrevatykh IuA, Fediukina DV, Filimonov VV Mol Biol (Mosk). 2010 Nov-Dec;44(6):1064-74. PMID:21290828^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kutyshenko VP, Gushchina LV, Khristoforov VS, Prokhorov DA, Timchenko MA, Kudrevatykh IuA, Fediukina DV, Filimonov VV. [NMR structure and dynamics of the chimeric protein SH3-F2]. Mol Biol (Mosk). 2010 Nov-Dec;44(6):1064-74. PMID:21290828

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kxd"

@@ Line 1: / Line 1: @@
 ==The structure of SH3-F2==
-<StructureSection load='2kxd' size='340' side='right'caption='[[2kxd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2kxd' size='340' side='right'caption='[[2kxd]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kxd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KXD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kxd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KXD FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jmc|2jmc]], [[1tuc|1tuc]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kxd OCA], [https://pdbe.org/2kxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kxd RCSB], [https://www.ebi.ac.uk/pdbsum/2kxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kxd ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPTAN1, SPTA2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kxd OCA], [https://pdbe.org/2kxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kxd RCSB], [https://www.ebi.ac.uk/pdbsum/2kxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kxd ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Gallus gallus]]
 [[Category: Large Structures]]
-[[Category: Synthetic construct sequences]]
+[[Category: Synthetic construct]]
-[[Category: Fedyukina, D V]]
+[[Category: Fedyukina DV]]
-[[Category: Filimonov, V V]]
+[[Category: Filimonov VV]]
-[[Category: Gushchina, L V]]
+[[Category: Gushchina LV]]
-[[Category: Khristoforov, V S]]
+[[Category: Khristoforov VS]]
-[[Category: Kudrevatykh, I u.A]]
+[[Category: Kudrevatykh IuA]]
-[[Category: Kutyshenko, V P]]
+[[Category: Kutyshenko VP]]
-[[Category: Prokhorov, D A]]
+[[Category: Prokhorov DA]]
-[[Category: Timchenko, M A]]
+[[Category: Timchenko MA]]
-[[Category: Alpha spectrin sh3 domain]]
-[[Category: Signaling protein]]
-[[Category: Spc-s19p20s circular permutant]]

2kxd

From Proteopedia

Revision as of 08:07, 18 January 2023

The structure of SH3-F2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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