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| | ==Eimeria tenella microneme protein 3 MAR_B domain== | | ==Eimeria tenella microneme protein 3 MAR_B domain== |
| - | <StructureSection load='2lbo' size='340' side='right'caption='[[2lbo]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2lbo' size='340' side='right'caption='[[2lbo]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lbo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coccidian_parasite Coccidian parasite]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LBO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lbo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eimeria_tenella Eimeria tenella]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LBO FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mic3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5802 Coccidian parasite])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lbo OCA], [https://pdbe.org/2lbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lbo RCSB], [https://www.ebi.ac.uk/pdbsum/2lbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lbo ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lbo OCA], [https://pdbe.org/2lbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lbo RCSB], [https://www.ebi.ac.uk/pdbsum/2lbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lbo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B6VCV4_EIMTE B6VCV4_EIMTE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Coccidian parasite]] | |
| - | [[Category: Large Structures]] | |
| - | [[Category: Garnett, J]] | |
| - | [[Category: Lai, L]] | |
| - | [[Category: Matthews, S]] | |
| - | [[Category: Simpson, P]] | |
| - | [[Category: Apicomplexan]] | |
| - | [[Category: Cell adhesion]] | |
| | [[Category: Eimeria tenella]] | | [[Category: Eimeria tenella]] |
| - | [[Category: Microneme]] | + | [[Category: Large Structures]] |
| - | [[Category: Microneme adhesive repeat]] | + | [[Category: Garnett J]] |
| - | [[Category: Protozoa]] | + | [[Category: Lai L]] |
| | + | [[Category: Matthews S]] |
| | + | [[Category: Simpson P]] |
| Structural highlights
Function
B6VCV4_EIMTE
Publication Abstract from PubMed
Eimeria spp. are a highly successful group of intracellular protozoan parasites that develop within intestinal epithelial cells of poultry, causing coccidiosis. As a result of resistance against anticoccidial drugs and the expense of manufacturing live vaccines, it is necessary to understand the relationship between Eimeria and its host more deeply, with a view to developing recombinant vaccines. Eimeria possesses a family of microneme lectins (MICs) that contain microneme adhesive repeat regions (MARR). We show that the major MARR protein from Eimeria tenella, EtMIC3, is deployed at the parasite-host interface during the early stages of invasion. EtMIC3 consists of seven tandem MAR1-type domains, which possess a high specificity for sialylated glycans as shown by cell-based assays and carbohydrate microarray analyses. The restricted tissue staining pattern observed for EtMIC3 in the chicken caecal epithelium indicates that EtMIC3 contributes to guiding the parasite to the site of invasion in the chicken gut. The microarray analyses also reveal a lack of recognition of glycan sequences terminating in the N-glycolyl form of sialic acid by EtMIC3. Thus the parasite is well adapted to the avian host which lacks N-glycolyl neuraminic acid. We provide new structural insight into the MAR1 family of domains and reveal the atomic resolution basis for the sialic acid-based carbohydrate recognition. Finally, a preliminary chicken immunization trial provides evidence that recombinant EtMIC3 protein and EtMIC3 DNA are effective vaccine candidates.
The Role of Sialyl Glycan Recognition in Host Tissue Tropism of the Avian Parasite Eimeria tenella.,Lai L, Bumstead J, Liu Y, Garnett J, Campanero-Rhodes MA, Blake DP, Palma AS, Chai W, Ferguson DJ, Simpson P, Feizi T, Tomley FM, Matthews S PLoS Pathog. 2011 Oct;7(10):e1002296. Epub 2011 Oct 13. PMID:22022267[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lai L, Bumstead J, Liu Y, Garnett J, Campanero-Rhodes MA, Blake DP, Palma AS, Chai W, Ferguson DJ, Simpson P, Feizi T, Tomley FM, Matthews S. The Role of Sialyl Glycan Recognition in Host Tissue Tropism of the Avian Parasite Eimeria tenella. PLoS Pathog. 2011 Oct;7(10):e1002296. Epub 2011 Oct 13. PMID:22022267 doi:10.1371/journal.ppat.1002296
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