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| | ==Crystal structure of TvoVMA intein== | | ==Crystal structure of TvoVMA intein== |
| - | <StructureSection load='4o1s' size='340' side='right' caption='[[4o1s]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='4o1s' size='340' side='right'caption='[[4o1s]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4o1s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thevo Thevo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O1S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4o1s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_volcanium_GSS1 Thermoplasma volcanium GSS1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O1S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atpA, TV0051, TVG0054274 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273116 THEVO])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1s OCA], [https://pdbe.org/4o1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o1s RCSB], [https://www.ebi.ac.uk/pdbsum/4o1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o1s ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1s OCA], [http://pdbe.org/4o1s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o1s RCSB], [http://www.ebi.ac.uk/pdbsum/4o1s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o1s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VATA_THEVO VATA_THEVO]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit. | + | [https://www.uniprot.org/uniprot/VATA_THEVO VATA_THEVO] Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[ATPase|ATPase]] | + | *[[ATPase 3D structures|ATPase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thevo]] | + | [[Category: Large Structures]] |
| - | [[Category: Aranko, A S]] | + | [[Category: Thermoplasma volcanium GSS1]] |
| - | [[Category: Iwai, H]] | + | [[Category: Aranko AS]] |
| - | [[Category: Kajander, T]] | + | [[Category: Iwai H]] |
| - | [[Category: Oeemig, J S]] | + | [[Category: Kajander T]] |
| - | [[Category: Wlodawer, A]] | + | [[Category: Oeemig JS]] |
| - | [[Category: Zhou, D]] | + | [[Category: Wlodawer A]] |
| - | [[Category: Splicing]]
| + | [[Category: Zhou D]] |
| Structural highlights
Function
VATA_THEVO Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.
Publication Abstract from PubMed
Protein splicing is an autocatalytic process involving self-excision of an internal protein domain, the intein, and concomitant ligation of the two flanking sequences, the exteins, with a peptide bond. Protein splicing can also take place in trans by naturally split inteins or artificially split inteins, ligating the exteins on two different polypeptide chains into one polypeptide chain. Protein trans-splicing could work in foreign contexts by replacing the native extein sequences with other protein sequences. Protein ligation using protein trans-splicing increasingly becomes a useful tool for biotechnological applications such as semi-synthesis of proteins, segmental isotopic labeling, and in vivo protein engineering. However, only a few split inteins have been successfully applied for protein ligation. Naturally split inteins have been widely used, but they are cross-reactive to each other, limiting their applications to multiple-fragment ligation. Based on the three-dimensional structures including two newly determined intein structures, we derived 21 new split inteins from four highly efficient cis-splicing inteins, in order to develop novel split inteins suitable for protein ligation. We systematically compared trans-splicing of 24 split inteins and tested the cross-activities among them to identify orthogonal split intein fragments that could be used in chemical biology and biotechnological applications.
Structure-based engineering and comparison of novel split inteins for protein ligation.,Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H Mol Biosyst. 2014 Feb 26. PMID:24574026[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H. Structure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 Feb 26. PMID:24574026 doi:http://dx.doi.org/10.1039/c4mb00021h
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