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| | ==Crystal structure of CarG== | | ==Crystal structure of CarG== |
| - | <StructureSection load='4o7j' size='340' side='right' caption='[[4o7j]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='4o7j' size='340' side='right'caption='[[4o7j]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sers3 Sers3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_sp._ATCC_39006 Serratia sp. ATCC 39006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7J FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CarG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104623 SERS3])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [https://pdbe.org/4o7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [https://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [http://pdbe.org/4o7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [http://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Sers3]] | + | [[Category: Large Structures]] |
| - | [[Category: Luisi, B F]] | + | [[Category: Serratia sp. ATCC 39006]] |
| - | [[Category: Salmond, G P.C]]
| + | [[Category: Luisi BF]] |
| - | [[Category: Tichy, E M]] | + | [[Category: Salmond GPC]] |
| - | [[Category: Antibiotic resistance]] | + | [[Category: Tichy EM]] |
| - | [[Category: Carbapenem resistance]] | + | |
| - | [[Category: Lysozyme inhibitor structural homologue]]
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| Structural highlights
Publication Abstract from PubMed
In the Gram-negative enterobacterium Erwinia (Pectobacterium) and Serratia sp. ATCC 39006, intrinsic resistance to the carbapenem antibiotic 1-carbapen-2-em-3-carboxylic acid is mediated by the CarF and CarG proteins, by an unknown mechanism. Here, we report a high-resolution crystal structure for the Serratia sp. ATCC 39006 carbapenem resistance protein CarG. This structure of CarG is the first in the carbapenem intrinsic resistance (CIR) family of resistance proteins from carbapenem-producing bacteria. The crystal structure shows the protein to form a homodimer, in agreement with results from analytical gel filtration. The structure of CarG does not show homology with any known antibiotic resistance proteins nor does it belong to any well-characterised protein structural family. However, it is a close structural homologue of the bacterial inhibitor of invertebrate lysozyme, PliI-Ah, with some interesting structural variations, including the absence of the catalytic site responsible for lysozyme inhibition. Both proteins show a unique beta-sandwich fold with short terminal alpha-helices. The core of the protein is formed by stacked anti-parallel sheets that are individually very similar in the two proteins but differ in their packing interface, causing the splaying of the two sheets in CarG. Furthermore, a conserved cation binding site identified in CarG is absent from the homologue.
Crystal structure of the carbapenem intrinsic resistance protein CarG.,Tichy EM, Luisi BF, Salmond GP J Mol Biol. 2014 May 1;426(9):1958-70. doi: 10.1016/j.jmb.2014.02.016. Epub 2014 , Feb 28. PMID:24583229[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tichy EM, Luisi BF, Salmond GP. Crystal structure of the carbapenem intrinsic resistance protein CarG. J Mol Biol. 2014 May 1;426(9):1958-70. doi: 10.1016/j.jmb.2014.02.016. Epub 2014 , Feb 28. PMID:24583229 doi:http://dx.doi.org/10.1016/j.jmb.2014.02.016
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