7kp3
From Proteopedia
(Difference between revisions)
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==Adeno-associated virus serotype 5 at 2.1 Angstroms resolution, AAV5== | ==Adeno-associated virus serotype 5 at 2.1 Angstroms resolution, AAV5== | ||
| - | <StructureSection load='7kp3' size='340' side='right'caption='[[7kp3]]' scene=''> | + | <StructureSection load='7kp3' size='340' side='right'caption='[[7kp3]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KP3 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7kp3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_5 Adeno-associated virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KP3 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kp3 OCA], [https://pdbe.org/7kp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kp3 RCSB], [https://www.ebi.ac.uk/pdbsum/7kp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kp3 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9YIJ1_9VIRU Q9YIJ1_9VIRU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Adeno-Associated Virus is the leading vector for gene therapy. Although it is the vector for all in vivo gene therapies approved for clinical use by the US Food and Drug Administration, its biology is still not yet fully understood. It has been shown that different serotypes of AAV bind to their cellular receptor, AAVR, in different ways. Previously we have reported a 2.4A structure of AAV2 bound to AAVR that shows ordered structure for only one of the two AAVR domains with which AAV2 interacts. In this study we present a 2.5A resolution structure of AAV5 bound to AAVR. AAV5 binds to the first polycystic kidney disease (PKD) domain of AAVR that was not ordered in the AAV2 structure. Interactions of AAV5 with AAVR are analyzed in detail, and the implications for AAV2 binding are explored through molecular modeling. Moreover, we find that binding sites for the antibodies ADK5a, ADK5b, and 3C5 on AAV5 overlap with the binding site of AAVR. These insights provide a structural foundation for development of gene therapy agents to better evade immune neutralization without disrupting cellular entry. | ||
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| + | The Structure of an AAV5-AAVR Complex at 2.5 A Resolution: Implications for Cellular Entry and Immune Neutralization of AAV Gene Therapy Vectors.,Silveria MA, Large EE, Zane GM, White TA, Chapman MS Viruses. 2020 Nov 18;12(11). pii: v12111326. doi: 10.3390/v12111326. PMID:33218165<ref>PMID:33218165</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7kp3" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Adeno-associated virus 5]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chapman MS]] | [[Category: Chapman MS]] | ||
[[Category: Silveria M]] | [[Category: Silveria M]] | ||
Revision as of 06:53, 25 January 2023
Adeno-associated virus serotype 5 at 2.1 Angstroms resolution, AAV5
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