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| | <StructureSection load='4oln' size='340' side='right'caption='[[4oln]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='4oln' size='340' side='right'caption='[[4oln]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oln]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OLN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oln]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OLN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ond|4ond]], [[4oor|4oor]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oln OCA], [https://pdbe.org/4oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oln RCSB], [https://www.ebi.ac.uk/pdbsum/4oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oln ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oln OCA], [http://pdbe.org/4oln PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oln RCSB], [http://www.ebi.ac.uk/pdbsum/4oln PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oln ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Synthetic construct sequences]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Murphy, M N]] | + | [[Category: Murphy MN]] |
| - | [[Category: Ortlund, E O]] | + | [[Category: Ortlund EO]] |
| - | [[Category: Estrogen receptor]]
| + | |
| - | [[Category: Nuclear receptor zinc finger]]
| + | |
| - | [[Category: Steroid receptor dna binding]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| - | [[Category: Transcription-dna complex]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Complex gene regulatory networks require transcription factors (TFs) to bind distinct DNA sequences. To understand how novel TF specificity evolves, we combined phylogenetic, biochemical, and biophysical approaches to interrogate how DNA recognition diversified in the steroid hormone receptor (SR) family. After duplication of the ancestral SR, three mutations in one copy radically weakened binding to the ancestral estrogen response element (ERE) and improved binding to a new set of DNA sequences (steroid response elements, SREs). They did so by establishing unfavorable interactions with ERE and abolishing unfavorable interactions with SRE; also required were numerous permissive substitutions, which nonspecifically improved cooperativity and affinity of DNA binding. Our findings indicate that negative determinants of binding play key roles in TFs' DNA selectivity and-with our prior work on the evolution of SR ligand specificity during the same interval-show how a specific new gene regulatory module evolved without interfering with the integrity of the ancestral module.
Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module.,McKeown AN, Bridgham JT, Anderson DW, Murphy MN, Ortlund EA, Thornton JW Cell. 2014 Sep 25;159(1):58-68. doi: 10.1016/j.cell.2014.09.003. PMID:25259920[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ McKeown AN, Bridgham JT, Anderson DW, Murphy MN, Ortlund EA, Thornton JW. Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module. Cell. 2014 Sep 25;159(1):58-68. doi: 10.1016/j.cell.2014.09.003. PMID:25259920 doi:http://dx.doi.org/10.1016/j.cell.2014.09.003
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