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| | <StructureSection load='4osz' size='340' side='right'caption='[[4osz]], [[Resolution|resolution]] 2.61Å' scene=''> | | <StructureSection load='4osz' size='340' side='right'caption='[[4osz]], [[Resolution|resolution]] 2.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4osz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacterium_campestre_var._armoraciae Bacterium campestre var. armoraciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OSZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4osz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._armoraciae Xanthomonas campestris pv. armoraciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OSZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4osh|4osh]], [[4osi|4osi]], [[4osj|4osj]], [[4osk|4osk]], [[4osl|4osl]], [[4osm|4osm]], [[4osq|4osq]], [[4osr|4osr]], [[4oss|4oss]], [[4ost|4ost]], [[4osv|4osv]], [[4osw|4osw]], [[4ot0|4ot0]], [[4ot3|4ot3]], [[4oto|4oto]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4osz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4osz OCA], [https://pdbe.org/4osz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4osz RCSB], [https://www.ebi.ac.uk/pdbsum/4osz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4osz ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hax3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=329463 Bacterium campestre var. armoraciae])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4osz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4osz OCA], [http://pdbe.org/4osz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4osz RCSB], [http://www.ebi.ac.uk/pdbsum/4osz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4osz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q3ZD72_XANCA Q3ZD72_XANCA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium campestre var. armoraciae]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Deng, D]] | + | [[Category: Xanthomonas campestris pv. armoraciae]] |
| - | [[Category: Pan, X J]] | + | [[Category: Deng D]] |
| - | [[Category: Wu, J P]] | + | [[Category: Pan XJ]] |
| - | [[Category: Yan, C Y]] | + | [[Category: Wu JP]] |
| - | [[Category: Yan, N]] | + | [[Category: Yan CY]] |
| - | [[Category: Dna]] | + | [[Category: Yan N]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Dna binding protein-dna complex]]
| + | |
| Structural highlights
Function
Q3ZD72_XANCA
Publication Abstract from PubMed
Transcription activator-like (TAL) effectors specifically bind to double stranded (ds) DNA through a central domain of tandem repeats. Each TAL effector (TALE) repeat comprises 33-35 amino acids and recognizes one specific DNA base through a highly variable residue at a fixed position in the repeat. Structural studies have revealed the molecular basis of DNA recognition by TALE repeats. Examination of the overall structure reveals that the basic building block of TALE protein, namely a helical hairpin, is one-helix shifted from the previously defined TALE motif. Here we wish to suggest a structure-based re-demarcation of the TALE repeat which starts with the residues that bind to the DNA backbone phosphate and concludes with the base-recognition hyper-variable residue. This new numbering system is consistent with the alpha-solenoid superfamily to which TALE belongs, and reflects the structural integrity of TAL effectors. In addition, it confers integral number of TALE repeats that matches the number of bound DNA bases. We then present fifteen crystal structures of engineered dHax3 variants in complex with target DNA molecules, which elucidate the structural basis for the recognition of bases adenine (A) and guanine (G) by reported or uncharacterized TALE codes. Finally, we analyzed the sequence-structure correlation of the amino acid residues within a TALE repeat. The structural analyses reported here may advance the mechanistic understanding of TALE proteins and facilitate the design of TALEN with improved affinity and specificity.
Revisiting the TALE repeat.,Deng D, Yan C, Wu J, Pan X, Yan N Protein Cell. 2014 Apr;5(4):297-306. doi: 10.1007/s13238-014-0035-2. Epub 2014, Mar 14. PMID:24622844[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Deng D, Yan C, Wu J, Pan X, Yan N. Revisiting the TALE repeat. Protein Cell. 2014 Apr;5(4):297-306. doi: 10.1007/s13238-014-0035-2. Epub 2014, Mar 14. PMID:24622844 doi:http://dx.doi.org/10.1007/s13238-014-0035-2
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