|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4oua' size='340' side='right'caption='[[4oua]], [[Resolution|resolution]] 2.76Å' scene=''> | | <StructureSection load='4oua' size='340' side='right'caption='[[4oua]], [[Resolution|resolution]] 2.76Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oua]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus_var._bisporus_h97 Agaricus bisporus var. bisporus h97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OUA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oua]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus_var._bisporus_H97 Agaricus bisporus var. bisporus H97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OUA FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAC:N-ACETYL-SERINE'>SAC</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAC:N-ACETYL-SERINE'>SAC</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oua OCA], [https://pdbe.org/4oua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oua RCSB], [https://www.ebi.ac.uk/pdbsum/4oua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oua ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosinase Tyrosinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.18.1 1.14.18.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oua OCA], [http://pdbe.org/4oua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oua RCSB], [http://www.ebi.ac.uk/pdbsum/4oua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oua ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| Line 20: |
Line 18: |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Tyrosinase|Tyrosinase]] | + | *[[Tyrosinase 3D structures|Tyrosinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Agaricus bisporus var. bisporus h97]] | + | [[Category: Agaricus bisporus var. bisporus H97]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tyrosinase]]
| + | [[Category: Al-Oweini R]] |
| - | [[Category: Al-Oweini, R]] | + | [[Category: Kortz U]] |
| - | [[Category: Kortz, U]] | + | [[Category: Molitor C]] |
| - | [[Category: Molitor, C]] | + | [[Category: Rompel A]] |
| - | [[Category: Rompel, A]] | + | [[Category: StMauracher G]] |
| - | [[Category: St Mauracher, G]] | + | |
| - | [[Category: Anderson-evans-type polyoxometalate]]
| + | |
| - | [[Category: Hetero-protein co-crystallization]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Ppo4]]
| + | |
| - | [[Category: Type-3 copper protein]]
| + | |
| - | [[Category: Tyrosinase maturation]]
| + | |
| - | [[Category: Zymogen]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Tyrosinases, bifunctional metalloenzymes, catalyze the oxidation of monophenols and o-diphenols to o-quinones, the precursor compounds of the brown-coloured pigment melanin. In eukaryotic organisms, tyrosinases are expressed as latent zymogens that have to be proteolytically cleaved in order to form highly active enzymes. This activation mechanism, known as the tyrosinase maturation process, has scientific and industrial significance with respect to biochemical and technical applications of the enzyme. Here, not only the first crystal structure of the mushroom tyrosinase abPPO4 is presented in its active form (Ser2-Ser383) and in its 21 kDa heavier latent form (Ser2-Thr545), but furthermore the simultaneous presence of both forms within one single-crystal structure is shown. This allows for a simple approach to investigate the transition between these two forms. Isoform abPPO4 was isolated and extensively purified from the natural source (Agaricus bisporus), which contains a total of six polyphenol oxidases (PPOs). The enzyme formed crystals (diffracting to a resolution of 2.76 A) owing to the employment of the 6-tungstotellurate(VI) salt (Na6[TeW6O24].22H2O) as a cocrystallization agent. Two of these disc-shaped Anderson-type polyoxoanions [TeW6O24](6-) separate two asymmetric units comprising one crystallographic heterodimer of abPPO4, thus resulting in very interesting crystal packing.
Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal.,Mauracher SG, Molitor C, Al-Oweini R, Kortz U, Rompel A Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2301-15. doi:, 10.1107/S1399004714013777. Epub 2014 Aug 29. PMID:25195745[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Mauracher SG, Molitor C, Al-Oweini R, Kortz U, Rompel A. Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal. Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2301-15. doi:, 10.1107/S1399004714013777. Epub 2014 Aug 29. PMID:25195745 doi:http://dx.doi.org/10.1107/S1399004714013777
|
