4ov0

From Proteopedia

(Difference between revisions)

Revision as of 07:47, 25 January 2023

Structure of Bacteriorhdopsin Transferred from Amphipol A8-35 to a Lipidic Mesophase

Structural highlights

4ov0 is a 1 chain structure with sequence from Halobacterium salinarum NRC-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BACR_HALSA Light-driven proton pump.

Publication Abstract from PubMed

Amphipols (APols) have become important tools for the stabilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs solubilized in APols would be of high importance for structural biology. However, despite considerable efforts, it is still not clear whether MP/APol complexes can form well-ordered crystals suitable for X-ray crystallography. In the present work, we show that an APol-trapped MP can be crystallized in meso. Bacteriorhodopsin (BR) trapped by APol A8-35 was mixed with a lipidic mesophase, and crystallization was induced by adding a precipitant. The crystals diffract beyond 2 A. The structure of BR was solved to 2 A and found to be indistinguishable from previous structures obtained after transfer from detergent solutions. We suggest the proposed protocol of in meso crystallization to be generally applicable to APol-trapped MPs.

High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase.,Polovinkin V, Gushchin I, Sintsov M, Round E, Balandin T, Chervakov P, Schevchenko V, Utrobin P, Popov A, Borshchevskiy V, Mishin A, Kuklin A, Willbold D, Chupin V, Popot JL, Gordeliy V J Membr Biol. 2014 Sep 6. PMID:25192977^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Polovinkin V, Gushchin I, Sintsov M, Round E, Balandin T, Chervakov P, Schevchenko V, Utrobin P, Popov A, Borshchevskiy V, Mishin A, Kuklin A, Willbold D, Chupin V, Popot JL, Gordeliy V. High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase. J Membr Biol. 2014 Sep 6. PMID:25192977 doi:http://dx.doi.org/10.1007/s00232-014-9700-x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ov0"

@@ Line 3: / Line 3: @@
 <StructureSection load='4ov0' size='340' side='right'caption='[[4ov0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ov0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halobacterium_sp. Halobacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OV0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ov0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OV0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ov0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ov0 OCA], [http://pdbe.org/4ov0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ov0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ov0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ov0 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ov0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ov0 OCA], [https://pdbe.org/4ov0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ov0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ov0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ov0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA]] Light-driven proton pump.
+[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Halobacterium sp]]
+[[Category: Halobacterium salinarum NRC-1]]
 [[Category: Large Structures]]
-[[Category: Balandin, T]]
+[[Category: Balandin T]]
-[[Category: Borshchevskiy, V]]
+[[Category: Borshchevskiy V]]
-[[Category: Chervakov, P]]
+[[Category: Chervakov P]]
-[[Category: Gordeliy, V]]
+[[Category: Gordeliy V]]
-[[Category: Gushchin, I]]
+[[Category: Gushchin I]]
-[[Category: Kuklin, A]]
+[[Category: Kuklin A]]
-[[Category: Mishin, A]]
+[[Category: Mishin A]]
-[[Category: Polovinkin, V]]
+[[Category: Polovinkin V]]
-[[Category: Popot, J L]]
+[[Category: Popot JL]]
-[[Category: Popov, A]]
+[[Category: Popov A]]
-[[Category: Round, E]]
+[[Category: Round E]]
-[[Category: Schevchenko, V]]
+[[Category: Schevchenko V]]
-[[Category: Sintsov, M]]
+[[Category: Sintsov M]]
-[[Category: Utrobin, P]]
+[[Category: Utrobin P]]
-[[Category: Willbold, D]]
+[[Category: Willbold D]]
-[[Category: Light-driven proton pump]]
-[[Category: Membrane]]
-[[Category: Retinal attached via schiff base]]
-[[Category: Transport protein]]

4ov0

From Proteopedia

Revision as of 07:47, 25 January 2023

Structure of Bacteriorhdopsin Transferred from Amphipol A8-35 to a Lipidic Mesophase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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