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| | <StructureSection load='4ov1' size='340' side='right'caption='[[4ov1]], [[Resolution|resolution]] 2.31Å' scene=''> | | <StructureSection load='4ov1' size='340' side='right'caption='[[4ov1]], [[Resolution|resolution]] 2.31Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ov1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhop2 Rhop2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OV1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ov1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopseudomonas_palustris_HaA2 Rhodopseudomonas palustris HaA2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OV1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPB_3630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316058 RHOP2])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ov1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ov1 OCA], [https://pdbe.org/4ov1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ov1 RCSB], [https://www.ebi.ac.uk/pdbsum/4ov1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ov1 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ov1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ov1 OCA], [http://pdbe.org/4ov1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ov1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ov1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ov1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2ITY5_RHOP2 Q2ITY5_RHOP2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Rhop2]] | + | [[Category: Rhodopseudomonas palustris HaA2]] |
| - | [[Category: Bell, S G]] | + | [[Category: Bell SG]] |
| - | [[Category: Wong, L L]] | + | [[Category: Wong L-L]] |
| - | [[Category: Zhang, A L]] | + | [[Category: Zhang AL]] |
| - | [[Category: Zhang, T]] | + | [[Category: Zhang T]] |
| - | [[Category: Zhouw, W H]] | + | [[Category: Zhouw WH]] |
| - | [[Category: 3fe-4s single cluster domain]]
| + | |
| - | [[Category: Electron transport]]
| + | |
| - | [[Category: Putative ferredoxin]]
| + | |
| Structural highlights
Function
Q2ITY5_RHOP2
Publication Abstract from PubMed
Rhodopseudomonas palustris HaA2 contains a gene, RPB3630, encoding a ferredoxin, HaPuxC, with an atypical CXXHXXC(X)nCP iron-sulfur cluster-binding motif. The ferredoxin gene is associated with a cytochrome P450 (CYP) monooxygenase-encoding gene, CYP194A3, an arrangement which is conserved in several strains of bacteria. Similar ferredoxin genes are found in other bacteria, such as Mycobacterium tuberculosis, where they are also associated with CYP genes. The crystal structure of HaPuxC has been solved at 2.3 A resolution. The overall fold of this [3Fe-4S] cluster-containing ferredoxin is similar to other [3Fe-4S] and [4Fe-4S] species, with the loop around the iron-sulfur cluster more closely resembling those of [3Fe-4S] ferredoxins. The side chain of His17 from the cluster-binding motif in HaPuxC points away from the vacant site of the cluster and interacts with Glu61 and one of the sulfide ions of the cluster. This is the first cytochrome P450 electron-transfer partner of this type to be structurally characterized and will provide a better understanding of the electron-transfer processes between these ferredoxins and their CYP enzymes.
The structure of a novel electron-transfer ferredoxin from Rhodopseudomonas palustris HaA2 which contains a histidine residue in its iron-sulfur cluster-binding motif.,Zhang T, Zhang A, Bell SG, Wong LL, Zhou W Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1453-64. doi:, 10.1107/S139900471400474X. Epub 2014 Apr 30. PMID:24816113[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang T, Zhang A, Bell SG, Wong LL, Zhou W. The structure of a novel electron-transfer ferredoxin from Rhodopseudomonas palustris HaA2 which contains a histidine residue in its iron-sulfur cluster-binding motif. Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1453-64. doi:, 10.1107/S139900471400474X. Epub 2014 Apr 30. PMID:24816113 doi:http://dx.doi.org/10.1107/S139900471400474X
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