1jqs

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[[Image:1jqs.gif|left|200px]]
[[Image:1jqs.gif|left|200px]]
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{{Structure
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{{STRUCTURE_1jqs| PDB=1jqs | SCENE= }}
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|RELATEDENTRY=[[1eg0|1EG0]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqs OCA], [http://www.ebi.ac.uk/pdbsum/1jqs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jqs RCSB]</span>
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'''Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog'''
'''Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog'''
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[[Category: Segupta, J.]]
[[Category: Segupta, J.]]
[[Category: 70s e coli ribosome]]
[[Category: 70s e coli ribosome]]
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[[Category: cryo-em]]
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[[Category: Cryo-em]]
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[[Category: ef-g]]
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[[Category: Ef-g]]
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[[Category: gtp state]]
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[[Category: Gtp state]]
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[[Category: l11]]
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[[Category: L11]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:41:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:37:38 2008''
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Revision as of 18:41, 2 May 2008

Image:1jqs.gif

Template:STRUCTURE 1jqs

Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog


Overview

L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation.

About this Structure

1JQS is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation., Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J, J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530 Page seeded by OCA on Fri May 2 21:41:04 2008

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