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| | <StructureSection load='4p1w' size='340' side='right'caption='[[4p1w]], [[Resolution|resolution]] 3.20Å' scene=''> | | <StructureSection load='4p1w' size='340' side='right'caption='[[4p1w]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4p1w]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_thermotolerans Kluyveromyces thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P1W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P1W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4p1w]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachancea_thermotolerans_CBS_6340 Lachancea thermotolerans CBS 6340]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P1W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P1W FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p1w OCA], [https://pdbe.org/4p1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p1w RCSB], [https://www.ebi.ac.uk/pdbsum/4p1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p1w ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4p1n|4p1n]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLTH0D11660g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans]), KLTH0C07942g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans]), KLTH0D15642g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p1w OCA], [http://pdbe.org/4p1w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p1w RCSB], [http://www.ebi.ac.uk/pdbsum/4p1w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p1w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C5DF24_LACTC C5DF24_LACTC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Kluyveromyces thermotolerans]] | + | [[Category: Lachancea thermotolerans CBS 6340]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fujioka, Y]] | + | [[Category: Fujioka Y]] |
| - | [[Category: Noda, N N]] | + | [[Category: Noda NN]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| Structural highlights
Function
C5DF24_LACTC
Publication Abstract from PubMed
Assembly of the preautophagosomal structure (PAS) is essential for autophagy initiation in yeast. Starvation-induced dephosphorylation of Atg13 is required for the formation of the Atg1-Atg13-Atg17-Atg29-Atg31 complex (Atg1 complex), a prerequisite for PAS assembly. However, molecular details underlying these events have not been established. Here we studied the interactions of yeast Atg13 with Atg1 and Atg17 by X-ray crystallography. Atg13 binds tandem microtubule interacting and transport domains in Atg1, using an elongated helix-loop-helix region. Atg13 also binds Atg17, using a short region, thereby bridging Atg1 and Atg17 and leading to Atg1-complex formation. Dephosphorylation of specific serines in Atg13 enhanced its interaction with not only Atg1 but also Atg17. These observations update the autophagy-initiation model as follows: upon starvation, dephosphorylated Atg13 binds both Atg1 and Atg17, and this promotes PAS assembly and autophagy progression.
Structural basis of starvation-induced assembly of the autophagy initiation complex.,Fujioka Y, Suzuki SW, Yamamoto H, Kondo-Kakuta C, Kimura Y, Hirano H, Akada R, Inagaki F, Ohsumi Y, Noda NN Nat Struct Mol Biol. 2014 May 4. doi: 10.1038/nsmb.2822. PMID:24793651[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Fujioka Y, Suzuki SW, Yamamoto H, Kondo-Kakuta C, Kimura Y, Hirano H, Akada R, Inagaki F, Ohsumi Y, Noda NN. Structural basis of starvation-induced assembly of the autophagy initiation complex. Nat Struct Mol Biol. 2014 May 4. doi: 10.1038/nsmb.2822. PMID:24793651 doi:http://dx.doi.org/10.1038/nsmb.2822
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