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| | <StructureSection load='4p9u' size='340' side='right'caption='[[4p9u]], [[Resolution|resolution]] 3.21Å' scene=''> | | <StructureSection load='4p9u' size='340' side='right'caption='[[4p9u]], [[Resolution|resolution]] 3.21Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4p9u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4p9u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P9U FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4p96|4p96]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9u OCA], [https://pdbe.org/4p9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p9u RCSB], [https://www.ebi.ac.uk/pdbsum/4p9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9u ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fadR, VC_1900 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9u OCA], [http://pdbe.org/4p9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p9u RCSB], [http://www.ebi.ac.uk/pdbsum/4p9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9u ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FADR_VIBCH FADR_VIBCH]] Multifunctional regulator of fatty acid metabolism. | + | [https://www.uniprot.org/uniprot/FADR_VIBCH FADR_VIBCH] Multifunctional regulator of fatty acid metabolism. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillo virgola del koch trevisan 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kull, F J]] | + | [[Category: Vibrio cholerae]] |
| - | [[Category: Shi, W]] | + | [[Category: Kull FJ]] |
| - | [[Category: Protein-dna complex]] | + | [[Category: Shi W]] |
| - | [[Category: Transcription regulator]]
| + | |
| - | [[Category: Transcription regulator-dna complex]]
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| Structural highlights
Function
FADR_VIBCH Multifunctional regulator of fatty acid metabolism.
Publication Abstract from PubMed
FadR is a master regulator of fatty acid metabolism and influences virulence in certain members of Vibrionaceae. Among FadR homologues of the GntR family, the Vibrionaceae protein is unusual in that it contains a C-terminal 40-residue insertion. Here we report the structure of Vibrio cholerae FadR (VcFadR) alone, bound to DNA, and in the presence of a ligand, oleoyl-CoA. Whereas Escherichia coli FadR (EcFadR) contains only one acyl-CoA-binding site in each monomer, crystallographic and calorimetric data indicate that VcFadR has two. One of the binding sites resembles that of EcFadR, whereas the other, comprised residues from the insertion, has not previously been observed. Upon ligand binding, VcFadR undergoes a dramatic conformational change that would more fully disrupt DNA binding than EcFadR. These findings suggest that the ability to bind and respond to an additional ligand allows FadR from Vibrionaceae to function as a more efficient regulator.
The 40-residue insertion in Vibrio cholerae FadR facilitates binding of an additional fatty acyl-CoA ligand.,Shi W, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ Nat Commun. 2015 Jan 21;6:6032. doi: 10.1038/ncomms7032. PMID:25607896[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shi W, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ. The 40-residue insertion in Vibrio cholerae FadR facilitates binding of an additional fatty acyl-CoA ligand. Nat Commun. 2015 Jan 21;6:6032. doi: 10.1038/ncomms7032. PMID:25607896 doi:http://dx.doi.org/10.1038/ncomms7032
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