This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4pcw

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 12:52, 1 February 2023

Crystal Structure of the N-terminal Domain of Human Profilaggrin at 2.2 A Resolution

Structural highlights

4pcw is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

FILA_HUMAN Autosomal dominant ichthyosis vulgaris. The disease is caused by mutations affecting the gene represented in this entry. Disease susceptibility is associated with variations affecting the gene represented in this entry.

Function

FILA_HUMAN Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Publication Abstract from PubMed

The fused-type S100 protein profilaggrin and its proteolytic products including filaggrin are important in the formation of a normal epidermal barrier; however, the specific function of the S100 calcium-binding domain in profilaggrin biology is poorly understood. To explore its molecular function, we determined a 2.2 A-resolution crystal structure of the N-terminal fused-type S100 domain of human profilaggrin with bound calcium ions. The profilaggrin S100 domain formed a stable dimer, which contained two hydrophobic pockets that provide a molecular interface for protein interactions. Biochemical and molecular approaches demonstrated that three proteins, annexin II/p36, stratifin/14-3-3 sigma, and Hsp27, bind to the N-terminal domain of human profilaggrin; one protein (stratifin) co-localized with profilaggrin in the differentiating granular cell layer of human skin. Together, these findings suggest a model where the profilaggrin N-terminus uses calcium-dependent and calcium-independent protein-protein interactions to regulate its involvement in keratinocyte terminal differentiation and incorporation into the cornified cell envelope.Journal of Investigative Dermatology accepted article preview online, 11 March 2015. doi:10.1038/jid.2015.102.

Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin and hsp27.,Bunick CG, Presland RB, Lawrence OT, Pearton DJ, Milstone LM, Steitz TA J Invest Dermatol. 2015 Mar 11. doi: 10.1038/jid.2015.102. PMID:25760235^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bunick CG, Presland RB, Lawrence OT, Pearton DJ, Milstone LM, Steitz TA. Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin and hsp27. J Invest Dermatol. 2015 Mar 11. doi: 10.1038/jid.2015.102. PMID:25760235 doi:http://dx.doi.org/10.1038/jid.2015.102

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4pcw"

Categories: Homo sapiens | Large Structures | Bunick CG | Steitz TA

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the N-terminal Domain of Human Profilaggrin at 2.2 A Resolution==
-<StructureSection load='4pcw' size='340' side='right' caption='[[4pcw]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='4pcw' size='340' side='right'caption='[[4pcw]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4pcw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4pcw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PCW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FLG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcw OCA], [https://pdbe.org/4pcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pcw RCSB], [https://www.ebi.ac.uk/pdbsum/4pcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcw ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcw OCA], [http://pdbe.org/4pcw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pcw RCSB], [http://www.ebi.ac.uk/pdbsum/4pcw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcw ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/FILA_HUMAN FILA_HUMAN]] Autosomal dominant ichthyosis vulgaris. The disease is caused by mutations affecting the gene represented in this entry.  Disease susceptibility is associated with variations affecting the gene represented in this entry.
+[https://www.uniprot.org/uniprot/FILA_HUMAN FILA_HUMAN] Autosomal dominant ichthyosis vulgaris. The disease is caused by mutations affecting the gene represented in this entry.  Disease susceptibility is associated with variations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/FILA_HUMAN FILA_HUMAN]] Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
+[https://www.uniprot.org/uniprot/FILA_HUMAN FILA_HUMAN] Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Bunick, C G]]
+[[Category: Large Structures]]
-[[Category: Steitz, T A]]
+[[Category: Bunick CG]]
-[[Category: Ef-hand calcium binding protein]]
+[[Category: Steitz TA]]
-[[Category: Epidermal skin protein]]
-[[Category: Metal binding protein]]
-[[Category: S100 protein]]
-[[Category: Signaling protein]]

4pcw

From Proteopedia

Revision as of 12:52, 1 February 2023

Crystal Structure of the N-terminal Domain of Human Profilaggrin at 2.2 A Resolution

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox