1jtp
From Proteopedia
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[[Image:1jtp.gif|left|200px]] | [[Image:1jtp.gif|left|200px]] | ||
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'''Degenerate interfaces in antigen-antibody complexes''' | '''Degenerate interfaces in antigen-antibody complexes''' | ||
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[[Category: Transue, T R.]] | [[Category: Transue, T R.]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
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| - | [[Category: | + | [[Category: Interface]] |
| - | [[Category: | + | [[Category: Vhh]] |
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Revision as of 18:54, 2 May 2008
Degenerate interfaces in antigen-antibody complexes
Overview
In most of the work dealing with the analysis of protein-protein interfaces, a single X-ray structure is available or selected, and implicitly it is assumed that this structure corresponds to the optimal complex for this pair of proteins. However, we have found a degenerate interface in a high-affinity antibody-antigen complex: the two independent complexes of the camel variable domain antibody fragment cAb-Lys3 and its antigen hen egg white lysozyme present in the asymmetric unit of our crystals show a difference in relative orientation between antibody and antigen, leading to important differences at the protein-protein interface. A third cAb-Lys3-hen lysozyme complex in a different crystal form adopts yet another relative orientation. Our results show that protein-protein interface characteristics can vary significantly between different specimens of the same high-affinity antibody-protein antigen complex. Consideration should be given to this type of observation when trying to establish general protein-protein interface characteristics.
About this Structure
1JTP is a Protein complex structure of sequences from Camelus dromedarius and Meleagris gallopavo. Full crystallographic information is available from OCA.
Reference
Degenerate interfaces in antigen-antibody complexes., Decanniere K, Transue TR, Desmyter A, Maes D, Muyldermans S, Wyns L, J Mol Biol. 2001 Oct 26;313(3):473-8. PMID:11676532 Page seeded by OCA on Fri May 2 21:54:28 2008
