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1jud

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[[Image:1jud.jpg|left|200px]]
[[Image:1jud.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1jud |SIZE=350|CAPTION= <scene name='initialview01'>1jud</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1jud", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CAT:Mutagenesis+Experiments+Have+Shown+That+These+Nine+Resid+...'>CAT</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-2-haloacid_dehalogenase (S)-2-haloacid dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.2 3.8.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= L-DEX YL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=286 Pseudomonas])
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-->
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|DOMAIN=
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{{STRUCTURE_1jud| PDB=1jud | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jud OCA], [http://www.ebi.ac.uk/pdbsum/1jud PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jud RCSB]</span>
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}}
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'''L-2-HALOACID DEHALOGENASE'''
'''L-2-HALOACID DEHALOGENASE'''
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==Reference==
==Reference==
Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp. YL. An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold., Hisano T, Hata Y, Fujii T, Liu JQ, Kurihara T, Esaki N, Soda K, J Biol Chem. 1996 Aug 23;271(34):20322-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8702766 8702766]
Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp. YL. An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold., Hisano T, Hata Y, Fujii T, Liu JQ, Kurihara T, Esaki N, Soda K, J Biol Chem. 1996 Aug 23;271(34):20322-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8702766 8702766]
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[[Category: (S)-2-haloacid dehalogenase]]
 
[[Category: Pseudomonas]]
[[Category: Pseudomonas]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Liu, J Q.]]
[[Category: Liu, J Q.]]
[[Category: Soda, K.]]
[[Category: Soda, K.]]
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[[Category: dehalogenase]]
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[[Category: Dehalogenase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:56:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:07 2008''
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Revision as of 18:56, 2 May 2008

Image:1jud.jpg

Template:STRUCTURE 1jud

L-2-HALOACID DEHALOGENASE


Overview

L-2-Haloacid dehalogenase catalyzes the hydrolytic dehalogenation of L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. The crystal structure of the homodimeric enzyme from Pseudomonas sp. YL has been determined by a multiple isomorphous replacement method and refined at 2.5 A resolution to a crystallographic R-factor of 19.5%. The subunit consists of two structurally distinct domains: the core domain and the subdomain. The core domain has an alpha/beta structure formed by a six-stranded parallel beta-sheet flanked by five alpha-helices. The subdomain inserted into the core domain has a four helix bundle structure providing the greater part of the interface for dimer formation. There is an active site cavity between the domains. An experimentally identified nucleophilic residue, Asp-10, is located on a loop following the amino-terminal beta-strand in the core domain, and other functional residues, Thr-14, Arg-41, Ser-118, Lys-151, Tyr-157, Ser-175, Asn-177, and Asp-180, detected by a site-directed mutagenesis experiment, are arranged around the nucleophile in the active site. Although the enzyme is an alpha/beta-type hydrolase, it does not belong to the alpha/beta hydrolase fold family, from the viewpoint of the topological feature and the position of the nucleophile.

About this Structure

1JUD is a Single protein structure of sequence from Pseudomonas. Full crystallographic information is available from OCA.

Reference

Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp. YL. An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold., Hisano T, Hata Y, Fujii T, Liu JQ, Kurihara T, Esaki N, Soda K, J Biol Chem. 1996 Aug 23;271(34):20322-30. PMID:8702766 Page seeded by OCA on Fri May 2 21:56:10 2008

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