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| | ==The crystal structure of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis== | | ==The crystal structure of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis== |
| - | <StructureSection load='4pon' size='340' side='right' caption='[[4pon]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4pon' size='340' side='right'caption='[[4pon]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pon]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacpz Bacpz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PON OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PON FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pon]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._spizizenii_str._W23 Bacillus subtilis subsp. spizizenii str. W23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PON FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4poo|4poo]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pon OCA], [https://pdbe.org/4pon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pon RCSB], [https://www.ebi.ac.uk/pdbsum/4pon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pon ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSUW23_14785, ytqB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=655816 BACPZ])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pon OCA], [http://pdbe.org/4pon PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pon RCSB], [http://www.ebi.ac.uk/pdbsum/4pon PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pon ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/E0TY72_BACSH E0TY72_BACSH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacpz]] | + | [[Category: Bacillus subtilis subsp. spizizenii str. W23]] |
| - | [[Category: Park, S C]] | + | [[Category: Large Structures]] |
| - | [[Category: Song, W S]] | + | [[Category: Park SC]] |
| - | [[Category: Yoon, S I]] | + | [[Category: Song WS]] |
| - | [[Category: A putative methyltransferase]] | + | [[Category: Yoon SI]] |
| - | [[Category: Rossmann-like fold]]
| + | |
| - | [[Category: S-adenosyl-l-methionine]]
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| - | [[Category: Transferase]]
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| Structural highlights
Function
E0TY72_BACSH
Publication Abstract from PubMed
S-adenosyl-l-methionine (SAM)-dependent methyltransferases (MTases) methylate diverse biological molecules using a SAM cofactor. The ytqB gene of Bacillus subtilis encodes a putative MTase and its biological function has never been characterized. To reveal the structural features and the cofactor binding mode of YtqB, we have determined the crystal structures of YtqB alone and in complex with its cofactor, SAM, at 1.9A and 2.2A resolutions, respectively. YtqB folds into a beta-sheet sandwiched by two alpha-helical layers, and assembles into a dimeric form. Each YtqB monomer contains one SAM binding site, which shapes SAM into a slightly curved conformation and exposes the reactive methyl group of SAM potentially to a substrate. Our comparative structural analysis of YtqB and its homologues indicates that YtqB is a SAM-dependent class I MTase, and provides insights into the substrate binding site of YtqB.
Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis.,Park SC, Song WS, Yoon SI Biochem Biophys Res Commun. 2014 Mar 14. pii: S0006-291X(14)00460-4. doi:, 10.1016/j.bbrc.2014.03.026. PMID:24637210[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Park SC, Song WS, Yoon SI. Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis. Biochem Biophys Res Commun. 2014 Mar 14. pii: S0006-291X(14)00460-4. doi:, 10.1016/j.bbrc.2014.03.026. PMID:24637210 doi:http://dx.doi.org/10.1016/j.bbrc.2014.03.026
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