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| | <StructureSection load='4ppj' size='340' side='right'caption='[[4ppj]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='4ppj' size='340' side='right'caption='[[4ppj]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ppj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PPJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PPJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ppj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PPJ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ppk|4ppk]], [[4ppl|4ppl]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ppj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ppj OCA], [https://pdbe.org/4ppj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ppj RCSB], [https://www.ebi.ac.uk/pdbsum/4ppj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ppj ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ppj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ppj OCA], [http://pdbe.org/4ppj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ppj RCSB], [http://www.ebi.ac.uk/pdbsum/4ppj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ppj ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Synthetic construct sequences]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Bell, T]] | + | [[Category: Bell T]] |
| - | [[Category: Bradbury, A]] | + | [[Category: Bradbury A]] |
| - | [[Category: Close, D]] | + | [[Category: Close D]] |
| - | [[Category: Devenish, R J]] | + | [[Category: Devenish RJ]] |
| - | [[Category: Paul, C Don]] | + | [[Category: Don Paul C]] |
| - | [[Category: Prescott, M]] | + | [[Category: Prescott M]] |
| - | [[Category: Traore, D A.K]] | + | [[Category: Traore DAK]] |
| - | [[Category: Wilce, M C.J]] | + | [[Category: Wilce MCJ]] |
| - | [[Category: Chromophore]]
| + | |
| - | [[Category: Chromoprotein]]
| + | |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Gfp]]
| + | |
| - | [[Category: Photoswitching]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Phanta is a reversibly photoswitching chromoprotein (PhiF, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (PhiF, 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in combination, to the optical properties of Phanta. Single amino acid substitutions at position 193 resulted in proteins with very low PhiF, indicating the importance of this position in controlling the fluorescence efficiency of the variant proteins. The substitution Thr69Val in Phanta was important for supressing the formation of a protonated chromophore species observed in some His193 substituted variants, whereas the substitution Gln62Met did not significantly contribute to the useful optical properties of Phanta. X-ray crystal structures for Phanta (2.3 A), eCGP123T69V (2.0 A) and eCGP123H193Q (2.2 A) in their non-photoswitched state were determined, revealing the presence of a cis-coplanar chromophore. We conclude that changes in the hydrogen-bonding network supporting the cis-chromophore, and its contacts with the surrounding protein matrix, are responsible for the low fluorescence emission of eCGP123 variants containing a His193 substitution.
X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein.,Don Paul C, Traore DA, Olsen S, Devenish RJ, Close DW, Bell TD, Bradbury A, Wilce MC, Prescott M PLoS One. 2015 Apr 29;10(4):e0123338. doi: 10.1371/journal.pone.0123338., eCollection 2015. PMID:25923520[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Don Paul C, Traore DA, Olsen S, Devenish RJ, Close DW, Bell TD, Bradbury A, Wilce MC, Prescott M. X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein. PLoS One. 2015 Apr 29;10(4):e0123338. doi: 10.1371/journal.pone.0123338., eCollection 2015. PMID:25923520 doi:http://dx.doi.org/10.1371/journal.pone.0123338
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