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| | <StructureSection load='4pq6' size='340' side='right'caption='[[4pq6]], [[Resolution|resolution]] 1.45Å' scene=''> | | <StructureSection load='4pq6' size='340' side='right'caption='[[4pq6]], [[Resolution|resolution]] 1.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pq6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phymc Phymc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQ6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pq6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PQ6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4it8|4it8]], [[4pqb|4pqb]], [[4pqc|4pqc]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pq6 OCA], [https://pdbe.org/4pq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pq6 RCSB], [https://www.ebi.ac.uk/pdbsum/4pq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pq6 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9755 PHYMC])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pq6 OCA], [http://pdbe.org/4pq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pq6 RCSB], [http://www.ebi.ac.uk/pdbsum/4pq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pq6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MYG_PHYCD MYG_PHYCD]] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. | + | [https://www.uniprot.org/uniprot/MYG_PHYMC MYG_PHYMC] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Myoglobin|Myoglobin]] | + | *[[Myoglobin 3D structures|Myoglobin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phymc]] | + | [[Category: Physeter catodon]] |
| - | [[Category: Wei, L]] | + | [[Category: Wei L]] |
| - | [[Category: Xiang-shi, T]] | + | [[Category: Xiang-shi T]] |
| - | [[Category: Ying-Wu, L]] | + | [[Category: Ying-Wu L]] |
| - | [[Category: Alpha helix bundle]]
| + | |
| - | [[Category: Enzyme function initiative]]
| + | |
| - | [[Category: Heme]]
| + | |
| - | [[Category: Nitrite reductase]]
| + | |
| - | [[Category: Oxygen transport]]
| + | |
| - | [[Category: Structural genomic]]
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| Structural highlights
Function
MYG_PHYMC Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Publication Abstract from PubMed
Heme coordination state determines the functional diversity of heme proteins. Using myoglobin as a model protein, we designed a distal hydrogen-bonding network by introducing both distal glutamic acid (Glu29) and histidine (His43) residues and regulated the heme into a bis-His coordination state with native ligands His64 and His93. This resembles the heme site in natural bis-His coordinated heme proteins such as cytoglobin and neuroglobin. A single mutation of L29E or F43H was found to form a distinct hydrogen-bonding network involving distal water molecules, instead of the bis-His heme coordination, which highlights the importance of the combination of multiple hydrogen-bonding interactions to regulate the heme coordination state. Kinetic studies further revealed that direct coordination of distal His64 to the heme iron negatively regulates fluoride binding and hydrogen peroxide activation by competing with the exogenous ligands. The new approach developed in this study can be generally applicable for fine-tuning the structure and function of heme proteins.
Regulating the coordination state of a heme protein by a designed distal hydrogen-bonding network.,Du JF, Li W, Li L, Wen GB, Lin YW, Tan X ChemistryOpen. 2015 Apr;4(2):97-101. doi: 10.1002/open.201402108. Epub 2014 Dec, 1. PMID:25969804[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Du JF, Li W, Li L, Wen GB, Lin YW, Tan X. Regulating the coordination state of a heme protein by a designed distal hydrogen-bonding network. ChemistryOpen. 2015 Apr;4(2):97-101. doi: 10.1002/open.201402108. Epub 2014 Dec, 1. PMID:25969804 doi:http://dx.doi.org/10.1002/open.201402108
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