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| | <StructureSection load='4pqq' size='340' side='right'caption='[[4pqq]], [[Resolution|resolution]] 1.55Å' scene=''> | | <StructureSection load='4pqq' size='340' side='right'caption='[[4pqq]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pqq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PQQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mkln1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqq OCA], [https://pdbe.org/4pqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pqq RCSB], [https://www.ebi.ac.uk/pdbsum/4pqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqq ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqq OCA], [http://pdbe.org/4pqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pqq RCSB], [http://www.ebi.ac.uk/pdbsum/4pqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MKLN1_MOUSE MKLN1_MOUSE]] Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1. | + | [https://www.uniprot.org/uniprot/MKLN1_MOUSE MKLN1_MOUSE] Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Hong, S K]] | + | [[Category: Hong SK]] |
| - | [[Category: Kim, E E]] | + | [[Category: Kim EE]] |
| - | [[Category: Kim, K H]] | + | [[Category: Kim K-H]] |
| - | [[Category: Alpha isoform]]
| + | |
| - | [[Category: Cell spreading mediator]]
| + | |
| - | [[Category: Jelly-roll]]
| + | |
| - | [[Category: Phosphorylation]]
| + | |
| - | [[Category: Prostaglandin ep3 receptor]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Ranbp9]]
| + | |
| Structural highlights
Function
MKLN1_MOUSE Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1.
Publication Abstract from PubMed
Muskelin is an intracellular kelch-repeat protein comprised of discoidin, LisH, CTLH and kelch-repeat domains. It is involved in cell adhesion and the regulation of cytoskeleton dynamics as well as being a component of a putative E3 ligase complex. Here, the first crystal structure of mouse muskelin discoidin domain (MK-DD) is reported at 1.55 A resolution, which reveals a distorted eight-stranded beta-barrel with two short alpha-helices at one end of the barrel. Interestingly, the N- and C-termini are not linked by the disulfide bonds found in other eukaryotic discoidin structures. A highly conserved MIND motif appears to be the determinant for MK-DD specific interaction together with the spike loops. Analysis of interdomain interaction shows that MK-DD binds the kelch-repeat domain directly and that this interaction depends on the presence of the LisH domain.
Structure of mouse muskelin discoidin domain and biochemical characterization of its self-association.,Kim KH, Hong SK, Hwang KY, Kim EE Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2863-74. doi:, 10.1107/S139900471401894X. Epub 2014 Oct 16. PMID:25372678[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim KH, Hong SK, Hwang KY, Kim EE. Structure of mouse muskelin discoidin domain and biochemical characterization of its self-association. Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2863-74. doi:, 10.1107/S139900471401894X. Epub 2014 Oct 16. PMID:25372678 doi:http://dx.doi.org/10.1107/S139900471401894X
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