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| | ==Crystal structure of swt1 C-terminal domain from yeast== | | ==Crystal structure of swt1 C-terminal domain from yeast== |
| - | <StructureSection load='4pqz' size='340' side='right' caption='[[4pqz]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4pqz' size='340' side='right'caption='[[4pqz]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pqz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pqz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PQZ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SWT1, YOR166C, O3595 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqz OCA], [https://pdbe.org/4pqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pqz RCSB], [https://www.ebi.ac.uk/pdbsum/4pqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqz OCA], [http://pdbe.org/4pqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pqz RCSB], [http://www.ebi.ac.uk/pdbsum/4pqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SWT1_YEAST SWT1_YEAST]] Involved in transcription. Probably functions as a transcriptional activator.<ref>PMID:16464826</ref> <ref>PMID:17030511</ref> | + | [https://www.uniprot.org/uniprot/SWT1_YEAST SWT1_YEAST] Involved in transcription. Probably functions as a transcriptional activator.<ref>PMID:16464826</ref> <ref>PMID:17030511</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Dong, Y H]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Gao, Z Q]] | + | [[Category: Dong YH]] |
| - | [[Category: Liu, Q S]] | + | [[Category: Gao ZQ]] |
| - | [[Category: Peng, S X]] | + | [[Category: Liu QS]] |
| - | [[Category: Hepn like fold]] | + | [[Category: Peng SX]] |
| - | [[Category: Transcription]]
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| Structural highlights
Function
SWT1_YEAST Involved in transcription. Probably functions as a transcriptional activator.[1] [2]
Publication Abstract from PubMed
Swt1 is an RNA endonuclease that plays an important role in quality control of nuclear messenger ribonucleoprotein particles (mRNPs) in eukaryotes; however, its structural details remain to be elucidated. Here, we report the crystal structure of the C-terminal (CT) domain of Swt1 from Saccharomyces cerevisiae, which shares common characteristics of higher eukaryotes and prokaryotes nucleotide binding (HEPN) domain superfamily. To study in detail the full-length protein structure, we analyzed the low-resolution architecture of Swt1 in solution using small angle X-ray scattering (SAXS) method. Both the CT domain and middle domain exhibited a good fit upon superimposing onto the molecular envelope of Swt1. Our study provides the necessary structural information for detailed analysis of the functional role of Swt1, and its importance in the process of nuclear mRNP surveillance.
High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S. cerevisiae RNA endonuclease Swt1.,Peng S, Zhou K, Wang W, Gao Z, Dong Y, Liu Q Biochem Biophys Res Commun. 2014 Oct 16;453(4):826-832. doi:, 10.1016/j.bbrc.2014.10.040. PMID:25450355[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Titz B, Thomas S, Rajagopala SV, Chiba T, Ito T, Uetz P. Transcriptional activators in yeast. Nucleic Acids Res. 2006 Feb 7;34(3):955-67. Print 2006. PMID:16464826 doi:http://dx.doi.org/34/3/955
- ↑ Rother S, Clausing E, Kieser A, Strasser K. Swt1, a novel yeast protein, functions in transcription. J Biol Chem. 2006 Dec 1;281(48):36518-25. Epub 2006 Oct 9. PMID:17030511 doi:http://dx.doi.org/M607510200
- ↑ Peng S, Zhou K, Wang W, Gao Z, Dong Y, Liu Q. High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S. cerevisiae RNA endonuclease Swt1. Biochem Biophys Res Commun. 2014 Oct 16;453(4):826-832. doi:, 10.1016/j.bbrc.2014.10.040. PMID:25450355 doi:http://dx.doi.org/10.1016/j.bbrc.2014.10.040
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