|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4pug' size='340' side='right'caption='[[4pug]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4pug' size='340' side='right'caption='[[4pug]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pug]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PUG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pug]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PUG FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4puh|4puh]], [[4pui|4pui]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pug OCA], [https://pdbe.org/4pug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pug RCSB], [https://www.ebi.ac.uk/pdbsum/4pug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pug ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AT1G55805, At1g55805 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pug OCA], [http://pdbe.org/4pug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pug RCSB], [http://www.ebi.ac.uk/pdbsum/4pug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pug ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/BOLA1_ARATH BOLA1_ARATH] May act either alone or in interaction with glutaredoxin as a redox-regulated transcriptional regulator, or as a factor regulating Fe-S cluster biogenesis (Probable). The glutaredoxin-BOLA1 heterodimers bind a labile, oxygen sensitive iron-sulfur cluster (PubMed:24714563).<ref>PMID:24714563</ref> <ref>PMID:24203231</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Didierjean, C]] | + | [[Category: Didierjean C]] |
| - | [[Category: Roret, T]] | + | [[Category: Roret T]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Morphogen]]
| + | |
| - | [[Category: Stress-responsive protein]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcriptional regulator]]
| + | |
| Structural highlights
Function
BOLA1_ARATH May act either alone or in interaction with glutaredoxin as a redox-regulated transcriptional regulator, or as a factor regulating Fe-S cluster biogenesis (Probable). The glutaredoxin-BOLA1 heterodimers bind a labile, oxygen sensitive iron-sulfur cluster (PubMed:24714563).[1] [2]
Publication Abstract from PubMed
BolA proteins are defined as stress-responsive transcriptional regulators but they also participate to iron metabolism. Although they can form [2Fe-2S]-containing complexes with monothiol glutaredoxins (Grx), structural details are lacking. Three Arabidopsis thaliana BolA structures were solved. They differ primarily by the size of a loop referred to as the variable [H/C] loop which contains an important cysteine (BolA_C group) or histidine (BolA_H group) residue. From 3D modeling and spectroscopic analyses of A. thaliana GrxS14-BolA1 holo-heterodimer (BolA_H), we provide evidence for the coordination of a Rieske-type [2Fe-2S] cluster. For BolA_C members, the cysteine could replace the histidine as a ligand. NMR interaction experiments using apo-proteins indicate that a completely different heterodimer was formed, involving the nucleic acid binding site of BolA and the C-terminal tail of Grx. The possible biological importance of these complexes is discussed considering the physiological functions previously assigned to BolA and to Grx-BolA or Grx-Grx complexes.
Structural and spectroscopic insights into BolA-glutaredoxin complexes.,Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C J Biol Chem. 2014 Jul 10. pii: jbc.M114.572701. PMID:25012657[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dhalleine T, Rouhier N, Couturier J. Putative roles of glutaredoxin-BolA holo-heterodimers in plants. Plant Signal Behav. 2014;9(3):e28564. PMID:24714563 doi:10.4161/psb.28564
- ↑ Couturier J, Wu HC, Dhalleine T, Pégeot H, Sudre D, Gualberto JM, Jacquot JP, Gaymard F, Vignols F, Rouhier N. Monothiol glutaredoxin-BolA interactions: redox control of Arabidopsis thaliana BolA2 and SufE1. Mol Plant. 2014 Jan;7(1):187-205. PMID:24203231 doi:10.1093/mp/sst156
- ↑ Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C. Structural and spectroscopic insights into BolA-glutaredoxin complexes. J Biol Chem. 2014 Jul 10. pii: jbc.M114.572701. PMID:25012657 doi:http://dx.doi.org/10.1074/jbc.M114.572701
|
