|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4pws' size='340' side='right'caption='[[4pws]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='4pws' size='340' side='right'caption='[[4pws]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pws]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PWS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pws]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PWS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT0046, mtc28, MTCY21D4.03c, Rv0040c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pws OCA], [https://pdbe.org/4pws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pws RCSB], [https://www.ebi.ac.uk/pdbsum/4pws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pws ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pws OCA], [http://pdbe.org/4pws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pws RCSB], [http://www.ebi.ac.uk/pdbsum/4pws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pws ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PR28_MYCTU PR28_MYCTU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 23: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Biswas, R]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Das, A K]] | + | [[Category: Biswas R]] |
| - | [[Category: Kundu, P]] | + | [[Category: Das AK]] |
| - | [[Category: Mueller-dieckmann, J]] | + | [[Category: Kundu P]] |
| - | [[Category: Mukherjee, S]] | + | [[Category: Mueller-dieckmann J]] |
| - | [[Category: Reinhard, L]] | + | [[Category: Mukherjee S]] |
| - | [[Category: Weiss, M S]] | + | [[Category: Reinhard L]] |
| - | [[Category: Immune system]]
| + | [[Category: Weiss MS]] |
| - | [[Category: Probable lipoprotein lpqn]]
| + | |
| Structural highlights
Function
PR28_MYCTU
Publication Abstract from PubMed
Secretary proteins of Mycobacterium tuberculosis are key players of the mycobacterial infection pathway. MTC28 is a 28-kDa proline-rich secretary antigen of Mycobacterium tuberculosis and is only conserved in pathogenic strains of mycobacteria. Here we report the crystal structure of MTC28 at 2.8- and 2.15-A resolutions for the structure-based epitope design. MTC28 shares a "mog1p"-fold consisting of seven antiparallel beta strands stacked between alpha helices. Five probable epitopes have been located on a solvent-accessible flexible region by computational analysis of the structure of MTC28. Simultaneously, the protein is digested with trypsin and the resulting fragments are purified by HPLC. Such 10 purified peptide fragments are screened against sera from patients infected with pulmonary tuberculosis (PTB). Two of these 10 fragments, namely (127)ALDITLPMPPR(137) and (138)WTQVPDPNVPDAFVVIADR(156),are found to be major immunogenic epitopes that are localized on the outer surface of the protein molecule and are part of a single continuous epitope that have been predicted in silico Mutagenesis and antibody inhibition studies are in accordance with the results obtained from epitope mapping.
Structure-based Epitope Mapping of Mycobacterium tuberculosis Secretary Antigen MTC28.,Kundu P, Biswas R, Mukherjee S, Reinhard L, Dutta A, Mueller-Dieckmann J, Weiss MS, Pal NK, Das AK J Biol Chem. 2016 Jul 1;291(27):13943-54. doi: 10.1074/jbc.M116.726422. Epub 2016, May 4. PMID:27189947[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kundu P, Biswas R, Mukherjee S, Reinhard L, Dutta A, Mueller-Dieckmann J, Weiss MS, Pal NK, Das AK. Structure-based Epitope Mapping of Mycobacterium tuberculosis Secretary Antigen MTC28. J Biol Chem. 2016 Jul 1;291(27):13943-54. doi: 10.1074/jbc.M116.726422. Epub 2016, May 4. PMID:27189947 doi:http://dx.doi.org/10.1074/jbc.M116.726422
|
