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| | <StructureSection load='4pxh' size='340' side='right'caption='[[4pxh]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='4pxh' size='340' side='right'caption='[[4pxh]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pxh]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._acta_2897 Streptomyces sp. acta 2897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PXH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PXH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pxh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._Acta_2897 Streptomyces sp. Acta 2897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PXH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=KH4:S-[2-({N-[(2R)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-BETA-ALANYL}AMINO)ETHYL]+1H-IMIDAZOLE-4-CARBOTHIOATE'>KH4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=KH4:S-[2-({N-[(2R)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-BETA-ALANYL}AMINO)ETHYL]+1H-IMIDAZOLE-4-CARBOTHIOATE'>KH4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4l0e|4l0e]], [[4l0f|4l0f]], [[4pwv|4pwv]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pxh OCA], [https://pdbe.org/4pxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pxh RCSB], [https://www.ebi.ac.uk/pdbsum/4pxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pxh ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sky32 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1001349 Streptomyces sp. Acta 2897]), sky30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1001349 Streptomyces sp. Acta 2897])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pxh OCA], [http://pdbe.org/4pxh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pxh RCSB], [http://www.ebi.ac.uk/pdbsum/4pxh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pxh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F2YRY7_9ACTN F2YRY7_9ACTN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Cytochrome P450|Cytochrome P450]] | + | *[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Streptomyces sp. acta 2897]] | + | [[Category: Streptomyces sp. Acta 2897]] |
| - | [[Category: Unspecific monooxygenase]]
| + | [[Category: Cryle MJ]] |
| - | [[Category: Cryle, M J]] | + | [[Category: Haslinger K]] |
| - | [[Category: Haslinger, K]] | + | |
| - | [[Category: Beta-aminoacyl carrier protein hydroxylase]]
| + | |
| - | [[Category: Cytochrome p450 fold]]
| + | |
| - | [[Category: Oxidoreductase-protein binding complex]]
| + | |
| - | [[Category: Peptidyl carrier protein domain]]
| + | |
| - | [[Category: Skyllamycin nrp]]
| + | |
| Structural highlights
Function
F2YRY7_9ACTN
Publication Abstract from PubMed
Studying the interplay between nonribosomal peptide synthetases (NRPS), a major source of secondary metabolites, and crucial external modifying enzymes is a challenging task since the interactions involved are often transient in nature. By applying a range of synthetic inhibitor-type compounds, a stabilized complex appropriate for structural analysis was generated for such a tailoring enzyme and an NRPS domain. The complex studied comprises an NRPS peptidyl carrier protein (PCP) domain bound to the Cytochrome P450 enzyme that is crucial for the provision of beta-hydroxylated amino acid precursors in the biosynthesis of the cyclic depsipeptide skyllamycin. The structure reveals that complex formation is governed by hydrophobic interactions, the presence of which can be controlled through minor alterations in PCP structure that enable selectivity amongst multiple highly similar PCP domains.
The structure of a transient complex of a nonribosomal Peptide synthetase and a cytochrome p450 monooxygenase.,Haslinger K, Brieke C, Uhlmann S, Sieverling L, Sussmuth RD, Cryle MJ Angew Chem Int Ed Engl. 2014 Aug 4;53(32):8518-22. doi: 10.1002/anie.201404977., Epub 2014 Jul 9. PMID:25044735[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Haslinger K, Brieke C, Uhlmann S, Sieverling L, Sussmuth RD, Cryle MJ. The structure of a transient complex of a nonribosomal Peptide synthetase and a cytochrome p450 monooxygenase. Angew Chem Int Ed Engl. 2014 Aug 4;53(32):8518-22. doi: 10.1002/anie.201404977., Epub 2014 Jul 9. PMID:25044735 doi:http://dx.doi.org/10.1002/anie.201404977
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