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| | ==The conserved ubiquitin-like protein hub1 plays a critical role in splicing in human cells== | | ==The conserved ubiquitin-like protein hub1 plays a critical role in splicing in human cells== |
| - | <StructureSection load='4pyu' size='340' side='right' caption='[[4pyu]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4pyu' size='340' side='right'caption='[[4pyu]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4pyu]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PYU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PYU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4pyu]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PYU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3plu|3plu]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pyu OCA], [https://pdbe.org/4pyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pyu RCSB], [https://www.ebi.ac.uk/pdbsum/4pyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pyu ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UBL5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pyu OCA], [http://pdbe.org/4pyu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pyu RCSB], [http://www.ebi.ac.uk/pdbsum/4pyu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pyu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SNUT1_HUMAN SNUT1_HUMAN]] Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.<ref>PMID:11350945</ref> | + | [https://www.uniprot.org/uniprot/UBL5_HUMAN UBL5_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Ammon, T]] | + | [[Category: Large Structures]] |
| - | [[Category: Holak, T A]] | + | [[Category: Ammon T]] |
| - | [[Category: Jentsch, S]] | + | [[Category: Holak TA]] |
| - | [[Category: Kowalska, K]] | + | [[Category: Jentsch S]] |
| - | [[Category: Mishra, S K]] | + | [[Category: Kowalska K]] |
| - | [[Category: Popowicz, G M]] | + | [[Category: Mishra SK]] |
| - | [[Category: Pre-mrna splicing]]
| + | [[Category: Popowicz GM]] |
| - | [[Category: Protein binding-allergen complex]]
| + | |
| - | [[Category: Ubiquitin-like]]
| + | |
| Structural highlights
Function
UBL5_HUMAN
Publication Abstract from PubMed
Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins non-covalently. In Saccharomyces cerevisiae, Hub1 associates with spliceosomes and mediates alternative splicing of SRC1, without affecting pre-mRNA splicing generally. Human Hub1 is highly similar to its yeast homolog, but its cellular function remains largely unexplored. Here, we show that human Hub1 binds to the spliceosomal protein Snu66 as in yeast; however, unlike its S. cerevisiae homolog, human Hub1 is essential for viability. Prolonged in vivo depletion of human Hub1 leads to various cellular defects, including splicing speckle abnormalities, partial nuclear retention of mRNAs, mitotic catastrophe, and consequently cell death by apoptosis. Early consequences of Hub1 depletion are severe splicing defects, however, only for specific splice sites leading to exon skipping and intron retention. Thus, the ubiquitin-like protein Hub1 is not a canonical spliceosomal factor needed generally for splicing, but rather a modulator of spliceosome performance and facilitator of alternative splicing.
The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells.,Ammon T, Mishra SK, Kowalska K, Popowicz GM, Holak TA, Jentsch S J Mol Cell Biol. 2014 Aug;6(4):312-23. doi: 10.1093/jmcb/mju026. Epub 2014 May, 28. PMID:24872507[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ammon T, Mishra SK, Kowalska K, Popowicz GM, Holak TA, Jentsch S. The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells. J Mol Cell Biol. 2014 Aug;6(4):312-23. doi: 10.1093/jmcb/mju026. Epub 2014 May, 28. PMID:24872507 doi:http://dx.doi.org/10.1093/jmcb/mju026
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