This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4q0k

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:39, 8 February 2023

Crystal Structure of Phytohormone Binding Protein from Medicago truncatula in complex with gibberellic acid (GA3)

Structural highlights

4q0k is a 1 chain structure with sequence from Medicago truncatula. This structure supersedes the now removed PDB entry 3us7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PHBP_MEDTR Binds gibberellin A3 (GA3) in vitro.^[1]

Publication Abstract from PubMed

Pathogenesis-related proteins of class 10 (PR-10) are a family of plant proteins with the same fold characterized by a large hydrophobic cavity that allows them to bind various ligands, such as phytohormones. A subfamily with only approximately 20% sequence identity but with a conserved canonical PR-10 fold have previously been recognized as Cytokinin-Specific Binding Proteins (CSBPs), although structurally the binding mode of trans-zeatin (a cytokinin phytohormone) was found to be quite diversified. Here, it is shown that two CSBP orthologues from Medicago truncatula and Vigna radiata bind gibberellic acid (GA3), which is an entirely different phytohormone, in a conserved and highly specific manner. In both cases a single GA3 molecule is found in the internal cavity of the protein. The structural data derived from high-resolution crystal structures are corroborated by isothermal titration calorimetry (ITC), which reveals a much stronger interaction with GA3 than with trans-zeatin and pH dependence of the binding profile. As a conclusion, it is postulated that the CSBP subfamily of plant PR-10 proteins should be more properly linked with general phytohormone-binding properties and termed phytohormone-binding proteins (PhBP).

Specific binding of gibberellic acid by Cytokinin-Specific Binding Proteins: a new aspect of plant hormone-binding proteins with the PR-10 fold.,Ruszkowski M, Sliwiak J, Ciesielska A, Barciszewski J, Sikorski M, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2014 Jul 1;70(Pt 7):2032-41. doi:, 10.1107/S1399004714010578. Epub 2014 Jun 29. PMID:25004979^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ruszkowski M, Sliwiak J, Ciesielska A, Barciszewski J, Sikorski M, Jaskolski M. Specific binding of gibberellic acid by Cytokinin-Specific Binding Proteins: a new aspect of plant hormone-binding proteins with the PR-10 fold. Acta Crystallogr D Biol Crystallogr. 2014 Jul 1;70(Pt 7):2032-41. doi:, 10.1107/S1399004714010578. Epub 2014 Jun 29. PMID:25004979 doi:http://dx.doi.org/10.1107/S1399004714010578
↑ Ruszkowski M, Sliwiak J, Ciesielska A, Barciszewski J, Sikorski M, Jaskolski M. Specific binding of gibberellic acid by Cytokinin-Specific Binding Proteins: a new aspect of plant hormone-binding proteins with the PR-10 fold. Acta Crystallogr D Biol Crystallogr. 2014 Jul 1;70(Pt 7):2032-41. doi:, 10.1107/S1399004714010578. Epub 2014 Jun 29. PMID:25004979 doi:http://dx.doi.org/10.1107/S1399004714010578

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4q0k"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Phytohormone Binding Protein from Medicago truncatula in complex with gibberellic acid (GA3)==
-<StructureSection load='4q0k' size='340' side='right' caption='[[4q0k]], [[Resolution|resolution]] 1.34&Aring;' scene=''>
+<StructureSection load='4q0k' size='340' side='right'caption='[[4q0k]], [[Resolution|resolution]] 1.34&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4q0k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Barrel_medic Barrel medic]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3us7 3us7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q0K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q0K FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4q0k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3us7 3us7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q0K FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GA3:GIBBERELLIN+A3'>GA3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GA3:GIBBERELLIN+A3'>GA3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2flh|2flh]], [[4jhg|4jhg]], [[2qim|2qim]], [[1xdf|1xdf]], [[1ifv|1ifv]], [[1icx|1icx]], [[4psb|4psb]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q0k OCA], [https://pdbe.org/4q0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q0k RCSB], [https://www.ebi.ac.uk/pdbsum/4q0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q0k ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mtphbp, MTR_3g055120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3880 Barrel medic])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q0k OCA], [http://pdbe.org/4q0k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q0k RCSB], [http://www.ebi.ac.uk/pdbsum/4q0k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q0k ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PHBP_MEDTR PHBP_MEDTR] Binds gibberellin A3 (GA3) in vitro.<ref>PMID:25004979</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Barrel medic]]
+[[Category: Large Structures]]
-[[Category: Barciszewski, J]]
+[[Category: Medicago truncatula]]
-[[Category: Ciesielska, A]]
+[[Category: Barciszewski J]]
-[[Category: Jaskolski, M]]
+[[Category: Ciesielska A]]
-[[Category: Ruszkowski, M]]
+[[Category: Jaskolski M]]
-[[Category: Sikorski, M]]
+[[Category: Ruszkowski M]]
-[[Category: Gibberellin]]
+[[Category: Sikorski M]]
-[[Category: Hormone binding protein]]
-[[Category: Plant hormone binding]]
-[[Category: Pr-10 fold]]

4q0k

From Proteopedia

Revision as of 07:39, 8 February 2023

Crystal Structure of Phytohormone Binding Protein from Medicago truncatula in complex with gibberellic acid (GA3)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox