This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


7xhx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 7xhx is ON HOLD until 2024-04-11
+
==Crystal structure of metallo-beta-lactamase IMP-6==
 +
<StructureSection load='7xhx' size='340' side='right'caption='[[7xhx]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[7xhx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6lvj 6lvj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHX FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhx OCA], [https://pdbe.org/7xhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhx RCSB], [https://www.ebi.ac.uk/pdbsum/7xhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhx ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/K4PWX3_ECOLX K4PWX3_ECOLX]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
IMP-type metallo-beta-lactamases confer resistance to carbapenems and a broad spectrum of beta-lactam antibiotics. IMP-6 and IMP-1 differ by only a point mutation: Ser262 in IMP-1 and Gly262 in IMP-6. The kcat/Km values of IMP-1 for imipenem and meropenem are nearly identical; however, for IMP-6, the kcat/Km for meropenem is 7-fold that for imipenem. In clinical practice, this may result in an ineffective therapeutic regimen and, consequently, in treatment failure. Here, we report the crystal structures of IMP-6 and IMP-1 with the same space group and similar cell constants at resolutions of 1.70 and 1.94 A, respectively. The overall structures of IMP-6 and IMP-1 are similar. However, the loop region (residues 60-66), which participates in substrate binding, is more flexible in IMP-6 than in IMP-1. This difference in flexibility determines the substrate specificity of IMP-type metallo-beta-lactamases for imipenem and meropenem. The amino acid at position 262 alters the mobility of His263; this affects the flexibility of the loop via a hydrogen bond with Pro68, which plays the role of a hinge in IMP-type metallo-beta-lactamases. The substitution of Pro68 with a glycine elicited an increase in the Km of IMP-6 for imipenem, whereas the affinity for meropenem remained unchanged.
-
Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H.
+
Structural insights into the substrate specificity of IMP-6 and IMP-1 metallo-beta-lactamases.,Yamamoto K, Tanaka H, Kurisu G, Nakano R, Yano H, Sakai H J Biochem. 2022 Dec 27;173(1):21-30. doi: 10.1093/jb/mvac080. PMID:36174533<ref>PMID:36174533</ref>
-
Description: Crystal structure of metallo-beta-lactamase IMP-6
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Yamamoto, K]]
+
<div class="pdbe-citations 7xhx" style="background-color:#fffaf0;"></div>
-
[[Category: Tanaka, H]]
+
== References ==
-
[[Category: Kurisu, G]]
+
<references/>
-
[[Category: Sakai, H]]
+
__TOC__
-
[[Category: Nakano, R]]
+
</StructureSection>
-
[[Category: Yano, H]]
+
[[Category: Escherichia coli]]
 +
[[Category: Large Structures]]
 +
[[Category: Kurisu G]]
 +
[[Category: Nakano R]]
 +
[[Category: Sakai H]]
 +
[[Category: Tanaka H]]
 +
[[Category: Yamamoto K]]
 +
[[Category: Yano H]]

Revision as of 10:17, 15 February 2023

Crystal structure of metallo-beta-lactamase IMP-6

PDB ID 7xhx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7xhx"
Personal tools