7xh9
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a dimeric interlocked parallel G-quadruplex== | |
| + | <StructureSection load='7xh9' size='340' side='right'caption='[[7xh9]], [[Resolution|resolution]] 1.63Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7xh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XH9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xh9 OCA], [https://pdbe.org/7xh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xh9 RCSB], [https://www.ebi.ac.uk/pdbsum/7xh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xh9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | 93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar concentration. Previous structural analyses of 93del using NMR spectroscopy have shown that the aptamer forms an interlocked G-quadruplex structure in K(+) solution. Due to its exceptional stability and unique topology, 93del has been used in many different studies involving DNA G-quadruplexes, such as DNA aptamer and multimer design, as well as DNA fluorescence research. To gain further insights on the structure of this unique aptamer, we have determined several high-resolution crystal structures of 93del and its variants. While confirming the overall dimeric interlocked G-quadruplex folding topology previously determined by NMR, our results reveal important detailed structural information, particularly the formation of a water-mediated A*G*G*G*G pentad. These insights allow us to better understand the formation of various structural elements in G-quadruplexes and should be useful for designing and manipulating G-quadruplex scaffolds with desired properties. | ||
| - | + | Crystal structures of an HIV-1 integrase aptamer: Formation of a water-mediated A*G*G*G*G pentad in an interlocked G-quadruplex.,Ngo KH, Liew CW, Lattmann S, Winnerdy FR, Phan AT Biochem Biophys Res Commun. 2022 Jul 12;613:153-158. doi: , 10.1016/j.bbrc.2022.04.020. Epub 2022 Apr 10. PMID:35561583<ref>PMID:35561583</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Lattmann | + | <div class="pdbe-citations 7xh9" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Lattmann S]] | ||
| + | [[Category: Liew CW]] | ||
| + | [[Category: Ngo KH]] | ||
| + | [[Category: Phan AT]] | ||
| + | [[Category: Winnerdy FR]] | ||
Revision as of 10:24, 15 February 2023
Crystal structure of a dimeric interlocked parallel G-quadruplex
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