7xhd

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'''Unreleased structure'''
 
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The entry 7xhd is ON HOLD until Paper Publication
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==Crystal structure of a dimeric interlocked parallel G-quadruplex==
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<StructureSection load='7xhd' size='340' side='right'caption='[[7xhd]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7xhd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHD FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhd OCA], [https://pdbe.org/7xhd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhd RCSB], [https://www.ebi.ac.uk/pdbsum/7xhd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhd ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar concentration. Previous structural analyses of 93del using NMR spectroscopy have shown that the aptamer forms an interlocked G-quadruplex structure in K(+) solution. Due to its exceptional stability and unique topology, 93del has been used in many different studies involving DNA G-quadruplexes, such as DNA aptamer and multimer design, as well as DNA fluorescence research. To gain further insights on the structure of this unique aptamer, we have determined several high-resolution crystal structures of 93del and its variants. While confirming the overall dimeric interlocked G-quadruplex folding topology previously determined by NMR, our results reveal important detailed structural information, particularly the formation of a water-mediated A*G*G*G*G pentad. These insights allow us to better understand the formation of various structural elements in G-quadruplexes and should be useful for designing and manipulating G-quadruplex scaffolds with desired properties.
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Authors: Ngo, K.H., Liew, C.W., Lattmann, S., Winnerdy, F.R., Phan, A.T.
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Crystal structures of an HIV-1 integrase aptamer: Formation of a water-mediated A*G*G*G*G pentad in an interlocked G-quadruplex.,Ngo KH, Liew CW, Lattmann S, Winnerdy FR, Phan AT Biochem Biophys Res Commun. 2022 Jul 12;613:153-158. doi: , 10.1016/j.bbrc.2022.04.020. Epub 2022 Apr 10. PMID:35561583<ref>PMID:35561583</ref>
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Description: Crystal structure of a dimeric interlocked parallel G-quadruplex
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Ngo, K.H]]
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<div class="pdbe-citations 7xhd" style="background-color:#fffaf0;"></div>
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[[Category: Liew, C.W]]
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== References ==
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[[Category: Lattmann, S]]
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<references/>
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[[Category: Phan, A.T]]
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__TOC__
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[[Category: Winnerdy, F.R]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Lattmann S]]
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[[Category: Liew CW]]
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[[Category: Ngo KH]]
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[[Category: Phan AT]]
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[[Category: Winnerdy FR]]

Revision as of 10:24, 15 February 2023

Crystal structure of a dimeric interlocked parallel G-quadruplex

PDB ID 7xhd

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