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2lev

From Proteopedia

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Revision as of 10:55, 15 February 2023

Structure of the DNA complex of the C-Terminal domain of Ler

Structural highlights

2lev is a 3 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7DB51_ECO57

Publication Abstract from PubMed

Ler, a member of the H-NS protein family, is the master regulator of the LEE pathogenicity island in virulent Escherichia coli strains. Here, we determined the structure of a complex between the DNA-binding domain of Ler (CT-Ler) and a 15-mer DNA duplex. CT-Ler recognizes a preexisting structural pattern in the DNA minor groove formed by two consecutive regions which are narrower and wider, respectively, compared with standard B-DNA. The compressed region, associated with an AT-tract, is sensed by the side chain of Arg90, whose mutation abolishes the capacity of Ler to bind DNA. The expanded groove allows the approach of the loop in which Arg90 is located. This is the first report of an experimental structure of a DNA complex that includes a protein belonging to the H-NS family. The indirect readout mechanism not only explains the capacity of H-NS and other H-NS family members to modulate the expression of a large number of genes but also the origin of the specificity displayed by Ler. Our results point to a general mechanism by which horizontally acquired genes may be specifically recognized by members of the H-NS family.

Indirect DNA Readout by an H-NS Related Protein: Structure of the DNA Complex of the C-Terminal Domain of Ler.,Cordeiro TN, Schmidt H, Madrid C, Juarez A, Bernado P, Griesinger C, Garcia J, Pons M PLoS Pathog. 2011 Nov;7(11):e1002380. Epub 2011 Nov 17. PMID:22114557^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cordeiro TN, Schmidt H, Madrid C, Juarez A, Bernado P, Griesinger C, Garcia J, Pons M. Indirect DNA Readout by an H-NS Related Protein: Structure of the DNA Complex of the C-Terminal Domain of Ler. PLoS Pathog. 2011 Nov;7(11):e1002380. Epub 2011 Nov 17. PMID:22114557 doi:10.1371/journal.ppat.1002380

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lev"

@@ Line 1: / Line 1: @@
 ==Structure of the DNA complex of the C-Terminal domain of Ler==
-<StructureSection load='2lev' size='340' side='right'caption='[[2lev]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2lev' size='340' side='right'caption='[[2lev]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2lev]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2lev]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEV FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ler ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lev OCA], [https://pdbe.org/2lev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lev RCSB], [https://www.ebi.ac.uk/pdbsum/2lev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lev ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lev OCA], [https://pdbe.org/2lev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lev RCSB], [https://www.ebi.ac.uk/pdbsum/2lev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lev ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q7DB51_ECO57 Q7DB51_ECO57]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Bernado, P]]
+[[Category: Bernado P]]
-[[Category: Cordeiro, T N]]
+[[Category: Cordeiro TN]]
-[[Category: Garcia, J]]
+[[Category: Garcia J]]
-[[Category: Grisienger, C]]
+[[Category: Grisienger C]]
-[[Category: Juarez, A]]
+[[Category: Juarez A]]
-[[Category: Madrid, C]]
+[[Category: Madrid C]]
-[[Category: Pons, M]]
+[[Category: Pons M]]
-[[Category: Schimdt, H]]
+[[Category: Schimdt H]]
-[[Category: Arginine-minor-groove recognition]]
-[[Category: Horizontal gene transfer]]
-[[Category: Indirect readout]]
-[[Category: Nucleoid associated protein]]
-[[Category: Transcription regulator-dna complex]]

2lev

From Proteopedia

Revision as of 10:55, 15 February 2023

Structure of the DNA complex of the C-Terminal domain of Ler

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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