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| | ==Intramolecular regulation of the ETS Domain within ETV6 sequence R335 to R458== | | ==Intramolecular regulation of the ETS Domain within ETV6 sequence R335 to R458== |
| - | <StructureSection load='2lf8' size='340' side='right'caption='[[2lf8]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''> | + | <StructureSection load='2lf8' size='340' side='right'caption='[[2lf8]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lf8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LF8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lf8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LF8 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2lf7|2lf7]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lf8 OCA], [https://pdbe.org/2lf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lf8 RCSB], [https://www.ebi.ac.uk/pdbsum/2lf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lf8 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Etv6, mouse ETV6, Tel, Tel1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lf8 OCA], [https://pdbe.org/2lf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lf8 RCSB], [https://www.ebi.ac.uk/pdbsum/2lf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lf8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ETV6_MOUSE ETV6_MOUSE]] Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'.
| + | [https://www.uniprot.org/uniprot/ETV6_MOUSE ETV6_MOUSE] Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Graves, B J]]
| + | [[Category: Coyne III H]] |
| - | [[Category: Green, S M]]
| + | [[Category: Graves BJ]] |
| - | [[Category: III, H Coyne]] | + | [[Category: Green SM]] |
| - | [[Category: Mcintosh, L P]] | + | [[Category: Mcintosh LP]] |
| - | [[Category: Auto-inhibition]] | + | |
| - | [[Category: Transcription]] | + | |
| Structural highlights
Function
ETV6_MOUSE Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'.
Publication Abstract from PubMed
ETV6 (or TEL), a transcriptional repressor belonging to the ETS family, is frequently involved in chromosomal translocations linked with human cancers. It displays a DNA-binding mode distinct from other ETS proteins due to the presence of a self-associating PNT domain. In this study, we used NMR spectroscopy to dissect the structural and dynamic bases for the autoinhibition of ETV6 DNA binding by sequences C-terminal to its ETS domain. The C-terminal inhibitory domain (CID) contains two helices, H4 and H5, which sterically block the DNA-binding interface of the ETS domain. Importantly, these appended helices are only marginally stable as revealed by amide hydrogen exchange and (15)N relaxation measurements. The CID is thus poised to undergo a facile conformational change as required for DNA binding. The CID also dampens millisecond timescale motions of the ETS domain hypothesized to be critical for the recognition of specific ETS target sequences. This work illustrates the use of appended sequences on conserved structural domains to generate biological diversity and complements previous studies of the allosteric mechanism of ETS1 autoinhibition to reveal both common and divergent features underlying the regulation of DNA binding by ETS transcription factors.
Autoinhibition of ETV6 (TEL) DNA Binding: Appended Helices Sterically Block the ETS Domain.,Coyne HJ 3rd, De S, Okon M, Green SM, Bhachech N, Graves BJ, McIntosh LP J Mol Biol. 2012 Aug 3;421(1):67-84. Epub 2012 May 12. PMID:22584210[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Coyne HJ 3rd, De S, Okon M, Green SM, Bhachech N, Graves BJ, McIntosh LP. Autoinhibition of ETV6 (TEL) DNA Binding: Appended Helices Sterically Block the ETS Domain. J Mol Biol. 2012 Aug 3;421(1):67-84. Epub 2012 May 12. PMID:22584210 doi:10.1016/j.jmb.2012.05.010
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