|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Solution structure of AR55 in 50% HFIP== | | ==Solution structure of AR55 in 50% HFIP== |
| - | <StructureSection load='2low' size='340' side='right'caption='[[2low]], [[NMR_Ensembles_of_Models | 40 NMR models]]' scene=''> | + | <StructureSection load='2low' size='340' side='right'caption='[[2low]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2low]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LOW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2low]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LOW FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2lot|2lot]], [[2lou|2lou]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2low FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2low OCA], [https://pdbe.org/2low PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2low RCSB], [https://www.ebi.ac.uk/pdbsum/2low PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2low ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">APLNR, AGTRL1, APJ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2low FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2low OCA], [https://pdbe.org/2low PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2low RCSB], [https://www.ebi.ac.uk/pdbsum/2low PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2low ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/APJ_HUMAN APJ_HUMAN]] Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
| + | [https://www.uniprot.org/uniprot/APJ_HUMAN APJ_HUMAN] Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Langelaan, D N]] | + | [[Category: Langelaan DN]] |
| - | [[Category: Rainey, J K]] | + | [[Category: Rainey JK]] |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Function
APJ_HUMAN Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
Publication Abstract from PubMed
Detergent micelles are frequently employed as membrane mimetics for solution-state membrane protein nuclear magnetic resonance spectroscopy. Here we compare topology, structure, ps-ns time-scale dynamics, and hydrodynamics of a model protein with one transmembrane (TM) segment (residues 1-55 of the apelin receptor, APJ, a G-protein-coupled receptor) in three distinct, commonly used micellar environments. In each environment, two solvent-protected helical segments connected by a solvent-exposed kink were observed. The break in helical character at the kink was maintained in a helix-stabilizing fluorinated alcohol environment, implying that this structural feature is inherent. Molecular dynamics simulations also substantiate favorable self-assembly of compact protein-micelle complexes with a more dynamic, solvent-exposed kink. Despite the observed similarity in TM segment behavior, micelle-dependent differences were clear in the structure, dynamics, and compactness of the 30-residue, extramembrane N-terminal tail of the protein. This would affect intermolecular interactions and, correspondingly, the functional state of the membrane protein.
Preserved Transmembrane Segment Topology, Structure, and Dynamics in Disparate Micellar Environments.,Langelaan DN, Pandey A, Sarker M, Rainey JK J Phys Chem Lett. 2017 May 12:2381-2386. doi: 10.1021/acs.jpclett.7b00867. PMID:28492329[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Langelaan DN, Pandey A, Sarker M, Rainey JK. Preserved Transmembrane Segment Topology, Structure, and Dynamics in Disparate Micellar Environments. J Phys Chem Lett. 2017 May 12:2381-2386. doi: 10.1021/acs.jpclett.7b00867. PMID:28492329 doi:http://dx.doi.org/10.1021/acs.jpclett.7b00867
|
