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| | ==VRR_NUC domain protein== | | ==VRR_NUC domain protein== |
| - | <StructureSection load='4qbl' size='340' side='right' caption='[[4qbl]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4qbl' size='340' side='right'caption='[[4qbl]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4qbl]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Psywf Psywf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QBL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4qbl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Psychrobacter_sp._PRwf-1 Psychrobacter sp. PRwf-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QBL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qbl OCA], [https://pdbe.org/4qbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qbl RCSB], [https://www.ebi.ac.uk/pdbsum/4qbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qbl ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qbn|4qbn]], [[4qbo|4qbo]]</td></tr>
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| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PsycPRwf_1331 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=349106 PSYWF])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qbl OCA], [http://pdbe.org/4qbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qbl RCSB], [http://www.ebi.ac.uk/pdbsum/4qbl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qbl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A5WF35_PSYWF A5WF35_PSYWF] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Psywf]] | + | [[Category: Large Structures]] |
| - | [[Category: Li, J]] | + | [[Category: Psychrobacter sp. PRwf-1]] |
| - | [[Category: Pennell, S]] | + | [[Category: Li J]] |
| - | [[Category: Smerdon, S J]] | + | [[Category: Pennell S]] |
| - | [[Category: Hydrolase]] | + | [[Category: Smerdon SJ]] |
| - | [[Category: Nuclease]]
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| Structural highlights
Function
A5WF35_PSYWF
Publication Abstract from PubMed
FAN1 is a structure-selective DNA repair nuclease with 5' flap endonuclease activity, involved in the repair of interstrand DNA crosslinks. It is the only eukaryotic protein with a virus-type replication-repair nuclease ("VRR-Nuc") "module" that commonly occurs as a standalone domain in many bacteria and viruses. Crystal structures of three representatives show that they structurally resemble Holliday junction resolvases (HJRs), are dimeric in solution, and are able to cleave symmetric four-way junctions. In contrast, FAN1 orthologs are monomeric and cleave 5' flap structures in vitro, but not Holliday junctions. Modeling of the VRR-Nuc domain of FAN1 reveals that it has an insertion, which packs against the dimerization interface observed in the structures of the viral/bacterial VRR-Nuc proteins. We propose that these additional structural elements in FAN1 prevent dimerization and bias specificity toward flap structures.
FAN1 activity on asymmetric repair intermediates is mediated by an atypical monomeric virus-type replication-repair nuclease domain.,Pennell S, Declais AC, Li J, Haire LF, Berg W, Saldanha JW, Taylor IA, Rouse J, Lilley DM, Smerdon SJ Cell Rep. 2014 Jul 10;8(1):84-93. doi: 10.1016/j.celrep.2014.06.001. Epub 2014, Jun 26. PMID:24981866[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pennell S, Declais AC, Li J, Haire LF, Berg W, Saldanha JW, Taylor IA, Rouse J, Lilley DM, Smerdon SJ. FAN1 activity on asymmetric repair intermediates is mediated by an atypical monomeric virus-type replication-repair nuclease domain. Cell Rep. 2014 Jul 10;8(1):84-93. doi: 10.1016/j.celrep.2014.06.001. Epub 2014, Jun 26. PMID:24981866 doi:http://dx.doi.org/10.1016/j.celrep.2014.06.001
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