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| | <StructureSection load='4qbn' size='340' side='right'caption='[[4qbn]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='4qbn' size='340' side='right'caption='[[4qbn]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4qbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_phage_setp3 Salmonella phage setp3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QBN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4qbn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_phage_SETP3 Salmonella phage SETP3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QBN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qbl|4qbl]], [[4qbo|4qbo]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qbn OCA], [https://pdbe.org/4qbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qbn RCSB], [https://www.ebi.ac.uk/pdbsum/4qbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qbn ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qbn OCA], [http://pdbe.org/4qbn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qbn RCSB], [http://www.ebi.ac.uk/pdbsum/4qbn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qbn ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A3EZR7_9CAUD A3EZR7_9CAUD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Salmonella phage setp3]] | + | [[Category: Salmonella phage SETP3]] |
| - | [[Category: Li, J]] | + | [[Category: Li J]] |
| - | [[Category: Pennell, S]] | + | [[Category: Pennell S]] |
| - | [[Category: Smerdon, S J]] | + | [[Category: Smerdon SJ]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Nuclease]]
| + | |
| Structural highlights
Function
A3EZR7_9CAUD
Publication Abstract from PubMed
FAN1 is a structure-selective DNA repair nuclease with 5' flap endonuclease activity, involved in the repair of interstrand DNA crosslinks. It is the only eukaryotic protein with a virus-type replication-repair nuclease ("VRR-Nuc") "module" that commonly occurs as a standalone domain in many bacteria and viruses. Crystal structures of three representatives show that they structurally resemble Holliday junction resolvases (HJRs), are dimeric in solution, and are able to cleave symmetric four-way junctions. In contrast, FAN1 orthologs are monomeric and cleave 5' flap structures in vitro, but not Holliday junctions. Modeling of the VRR-Nuc domain of FAN1 reveals that it has an insertion, which packs against the dimerization interface observed in the structures of the viral/bacterial VRR-Nuc proteins. We propose that these additional structural elements in FAN1 prevent dimerization and bias specificity toward flap structures.
FAN1 activity on asymmetric repair intermediates is mediated by an atypical monomeric virus-type replication-repair nuclease domain.,Pennell S, Declais AC, Li J, Haire LF, Berg W, Saldanha JW, Taylor IA, Rouse J, Lilley DM, Smerdon SJ Cell Rep. 2014 Jul 10;8(1):84-93. doi: 10.1016/j.celrep.2014.06.001. Epub 2014, Jun 26. PMID:24981866[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pennell S, Declais AC, Li J, Haire LF, Berg W, Saldanha JW, Taylor IA, Rouse J, Lilley DM, Smerdon SJ. FAN1 activity on asymmetric repair intermediates is mediated by an atypical monomeric virus-type replication-repair nuclease domain. Cell Rep. 2014 Jul 10;8(1):84-93. doi: 10.1016/j.celrep.2014.06.001. Epub 2014, Jun 26. PMID:24981866 doi:http://dx.doi.org/10.1016/j.celrep.2014.06.001
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