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4qiq

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Revision as of 11:31, 15 February 2023

Crystal structure of D-xylose-proton symporter

Structural highlights

4qiq is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

XYLE_ECOLI Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).

Publication Abstract from PubMed

The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformations, including both the outward and inward-facing states. Here we report the crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the rocker-switch movement of the N-domain against the C-domain during the transport cycle. Using molecular dynamics simulation, and functional transport assays, we describe the movement of XylE that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133. This study addresses the structural basis for proton-coupled substrate transport and release mechanism for the sugar porter family of proteins.

Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE.,Wisedchaisri G, Park MS, Iadanza MG, Zheng H, Gonen T Nat Commun. 2014 Aug 4;5:4521. doi: 10.1038/ncomms5521. PMID:25088546^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wisedchaisri G, Park MS, Iadanza MG, Zheng H, Gonen T. Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE. Nat Commun. 2014 Aug 4;5:4521. doi: 10.1038/ncomms5521. PMID:25088546 doi:http://dx.doi.org/10.1038/ncomms5521

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4qiq"

@@ Line 3: / Line 3: @@
 <StructureSection load='4qiq' size='340' side='right'caption='[[4qiq]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4qiq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QIQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QIQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4qiq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QIQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QIQ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b4031, JW3991, xylE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qiq OCA], [https://pdbe.org/4qiq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qiq RCSB], [https://www.ebi.ac.uk/pdbsum/4qiq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qiq ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qiq OCA], [http://pdbe.org/4qiq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qiq RCSB], [http://www.ebi.ac.uk/pdbsum/4qiq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qiq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/XYLE_ECOLI XYLE_ECOLI]] Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).
+[https://www.uniprot.org/uniprot/XYLE_ECOLI XYLE_ECOLI] Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 20: @@
 ==See Also==
-*[[Symporter|Symporter]]
+*[[Symporter 3D structures|Symporter 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Gonen, T]]
+[[Category: Gonen T]]
-[[Category: Iadanza, M G]]
+[[Category: Iadanza MG]]
-[[Category: Park, M]]
+[[Category: Park M]]
-[[Category: Wisedchaisri, G]]
+[[Category: Wisedchaisri G]]
-[[Category: Zheng, H]]
+[[Category: Zheng H]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Sugar transporter]]
-[[Category: Transport protein]]

4qiq

From Proteopedia

Revision as of 11:31, 15 February 2023

Crystal structure of D-xylose-proton symporter

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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