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| | ==Structure of C. trachomatis CT441== | | ==Structure of C. trachomatis CT441== |
| - | <StructureSection load='4ql6' size='340' side='right' caption='[[4ql6]], [[Resolution|resolution]] 2.97Å' scene=''> | + | <StructureSection load='4ql6' size='340' side='right'caption='[[4ql6]], [[Resolution|resolution]] 2.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ql6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlt2 Chlt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ql6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis_434/Bu Chlamydia trachomatis 434/Bu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QL6 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CT441, CTL0700, tsp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=471472 CHLT2])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ql6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql6 OCA], [https://pdbe.org/4ql6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ql6 RCSB], [https://www.ebi.ac.uk/pdbsum/4ql6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql6 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql6 OCA], [http://pdbe.org/4ql6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ql6 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H3MDM0_CHLT2 A0A0H3MDM0_CHLT2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chlt2]] | + | [[Category: Chlamydia trachomatis 434/Bu]] |
| - | [[Category: Hansen, G]] | + | [[Category: Large Structures]] |
| - | [[Category: Hilgenfeld, R]] | + | [[Category: Hansen G]] |
| - | [[Category: Kohlmann, F]] | + | [[Category: Hilgenfeld R]] |
| - | [[Category: Chaperone]] | + | [[Category: Kohlmann F]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Protease]]
| + | |
| - | [[Category: Ser/lys/gln catalytic triad]]
| + | |
| Structural highlights
Function
A0A0H3MDM0_CHLT2
Publication Abstract from PubMed
Chlamydia trachomatis is the most prevalent cause of preventable blindness worldwide and a major reason for infectious infertility in females. Several bacterial factors have been implicated in the pathogenesis of C. trachomatis. Combining structural and mutational analysis, we have shown that the proteolytic function of CT441 depends on a conserved Ser/Lys/Gln catalytic triad and a functional substrate-binding site within a flexible PDZ (postsynaptic density of 95 kDa, discs large, and zonula occludens) domain. Previously, it has been suggested that CT441 is involved in modulating estrogen signaling responses of the host cell. Our results show that although in vitro CT441 exhibits proteolytic activity against SRAP1, a coactivator of estrogen receptor alpha, CT441-mediated SRAP1 degradation is not observed during the intracellular developmental cycle before host cells are lysed and infectious chlamydiae are released. Most compellingly, we have newly identified a chaperone activity of CT441, indicating a role of CT441 in prokaryotic protein quality control processes.
Structural basis of the proteolytic and chaperone activity of Chlamydia trachomatis CT441.,Kohlmann F, Shima K, Hilgenfeld R, Solbach W, Rupp J, Hansen G J Bacteriol. 2015 Jan 1;197(1):211-8. doi: 10.1128/JB.02140-14. Epub 2014 Oct 27. PMID:25349155[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kohlmann F, Shima K, Hilgenfeld R, Solbach W, Rupp J, Hansen G. Structural basis of the proteolytic and chaperone activity of Chlamydia trachomatis CT441. J Bacteriol. 2015 Jan 1;197(1):211-8. doi: 10.1128/JB.02140-14. Epub 2014 Oct 27. PMID:25349155 doi:http://dx.doi.org/10.1128/JB.02140-14
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