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| | <StructureSection load='4qmi' size='340' side='right'caption='[[4qmi]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='4qmi' size='340' side='right'caption='[[4qmi]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4qmi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QMI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4qmi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QMI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qmh|4qmh]], [[4qmj|4qmj]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmi OCA], [https://pdbe.org/4qmi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qmi RCSB], [https://www.ebi.ac.uk/pdbsum/4qmi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmi ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">chTOG, CKAP5, KIAA0097 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmi OCA], [http://pdbe.org/4qmi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qmi RCSB], [http://www.ebi.ac.uk/pdbsum/4qmi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CKAP5_HUMAN CKAP5_HUMAN]] Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles.<ref>PMID:12569123</ref> <ref>PMID:21646404</ref> | + | [https://www.uniprot.org/uniprot/CKAP5_HUMAN CKAP5_HUMAN] Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles.<ref>PMID:12569123</ref> <ref>PMID:21646404</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Currie, J D]] | + | [[Category: Currie JD]] |
| - | [[Category: Fox, J C]] | + | [[Category: Fox JC]] |
| - | [[Category: Howard, A E]] | + | [[Category: Howard AE]] |
| - | [[Category: Rogers, S L]] | + | [[Category: Rogers SL]] |
| - | [[Category: Slep, K C]] | + | [[Category: Slep KC]] |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Tog domain]]
| + | |
| Structural highlights
Function
CKAP5_HUMAN Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles.[1] [2]
Publication Abstract from PubMed
XMAP215 family members are potent microtubule (MT) polymerases, with mutants displaying reduced MT growth rates and aberrant spindle morphologies. XMAP215 proteins contain arrayed TOG domains that bind tubulin. Whether these TOG domains are architecturally equivalent is unknown. Here, we present crystal structures of TOG4 from Drosophila Msps and human ch-TOG. These TOG4 structures architecturally depart from the structures of TOG domains 1 and 2, revealing a conserved domain bend that predicts a novel engagement with alpha-tubulin. In vitro assays show differential tubulin-binding affinities across the TOG array, as well as differential effects on MT polymerization. We used Drosophila S2 cells depleted of endogenous Msps to assess the importance of individual TOG domains. While a TOG1-4 array largely rescues MT polymerization rates, mutating tubulin-binding determinants in any single TOG domain dramatically reduces rescue activity. Our work highlights the structurally diverse, yet positionally conserved TOG array that drives MT polymerization.
The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array.,Fox JC, Howard AE, Currie JD, Rogers SL, Slep KC Mol Biol Cell. 2014 Jun 25. pii: mbc.E13-08-0501. PMID:24966168[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gergely F, Draviam VM, Raff JW. The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells. Genes Dev. 2003 Feb 1;17(3):336-41. PMID:12569123 doi:http://dx.doi.org/10.1101/gad.245603
- ↑ van der Vaart B, Manatschal C, Grigoriev I, Olieric V, Gouveia SM, Bjelic S, Demmers J, Vorobjev I, Hoogenraad CC, Steinmetz MO, Akhmanova A. SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase. J Cell Biol. 2011 Jun 13;193(6):1083-99. Epub 2011 Jun 6. PMID:21646404 doi:10.1083/jcb.201012179
- ↑ Fox JC, Howard AE, Currie JD, Rogers SL, Slep KC. The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array. Mol Biol Cell. 2014 Jun 25. pii: mbc.E13-08-0501. PMID:24966168 doi:http://dx.doi.org/10.1091/mbc.E13-08-0501
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